In E. coli, transcription repression is essential in cellular functioning. However, its failure rates are non-negligible. We measured the leakiness rate of lacO3O1 promoter with single RNA sensitivity and its temperature dependence in live cells. After finding strong temperature dependence, we dissected the causes. While RNA polymerase numbers and kt, the rate of active transcription, vary weakly with temperature, the repression strength (dependent on number of repressors and binding and unbinding rates of repressors to the promoter) is heavily temperature dependent. We conclude that the lacO3O1 leakiness at low temperatures increases as the repression mechanism’s efficiency hampers. © Springer International Publishing AG 2016.
|Title of host publication||Computational Methods in Systems Biology|
|Subtitle of host publication||14th conference on Computational Methods in Systems Biology (CMSB2016). 21st - 23rd September 2016|
|Publication status||Published - 2016|
- MS2-GFP RNA detection
- Time-lapse confocal microscopy
Gonçalves, N., Oliveira, S. M. D., Fonseca, J. M., & Ribeiro, A. S. (2016). Temperature Dependence of Leakiness of Transcription Repression Mechanisms of Escherichia coli. In Computational Methods in Systems Biology: 14th conference on Computational Methods in Systems Biology (CMSB2016). 21st - 23rd September 2016 (Vol. 9859, pp. 341-342). Springer.