TY - JOUR
T1 - Tauroursodeoxycholic Acid Improves Motor Symptoms in a Mouse Model of Parkinson’s Disease
AU - Rosa, Alexandra Isabel
AU - Duarte-Silva, Sara
AU - Silva-Fernandes, Anabela
AU - Nunes, Maria João
AU - Carvalho, Andreia Neves
AU - Rodrigues, Elsa
AU - Gama, Maria João
AU - Rodrigues, Cecília Maria Pereira
AU - Maciel, Patrícia
AU - Castro-Caldas, Margarida
N1 - info:eu-repo/grantAgreement/FCT/3599-PPCDT/124452/PT#
info:eu-repo/grantAgreement/FCT/5876/147348/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F95855%2F2013/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F98023%2F2013/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91562%2F2012/PT#
POCI-01-0145-FEDER-007038
SFRH/BPD72891/2010
UMINHO/BI/248/2016
NORTE-01-0145-FEDER-000013
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Parkinson’s disease (PD) is characterized by severe motor symptoms, and currently there is no treatment that retards disease progression or reverses damage prior to the time of clinical diagnosis. Tauroursodeoxycholic acid (TUDCA) is neuroprotective in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD; however, its effect in PD motor symptoms has never been addressed. In the present work, an extensive behavior analysis was performed to better characterize the MPTP model of PD and to evaluate the effects of TUDCA in the prevention/improvement of mice phenotype. MPTP induced significant alterations in general motor performance paradigms, including increased latency in the motor swimming, adhesive removal and pole tests, as well as altered gait, foot dragging, and tremors. TUDCA administration, either before or after MPTP, significantly reduced the swimming latency, improved gait quality, and decreased foot dragging. Importantly, TUDCA was also effective in the prevention of typical parkinsonian symptoms such as spontaneous activity, ability to initiate movement and tremors. Accordingly, TUDCA prevented MPTP-induced decrease of dopaminergic fibers and ATP levels, mitochondrial dysfunction and neuroinflammation. Overall, MPTP-injected mice presented motor symptoms that are aggravated throughout time, resembling human parkinsonism, whereas PD motor symptoms were absent or mild in TUDCA-treated animals, and no aggravation was observed in any parameter. The thorough demonstration of improvement of PD symptoms together with the demonstration of the pathways triggered by TUDCA supports a subsequent clinical trial in humans and future validation of the application of this bile acid in PD.
AB - Parkinson’s disease (PD) is characterized by severe motor symptoms, and currently there is no treatment that retards disease progression or reverses damage prior to the time of clinical diagnosis. Tauroursodeoxycholic acid (TUDCA) is neuroprotective in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD; however, its effect in PD motor symptoms has never been addressed. In the present work, an extensive behavior analysis was performed to better characterize the MPTP model of PD and to evaluate the effects of TUDCA in the prevention/improvement of mice phenotype. MPTP induced significant alterations in general motor performance paradigms, including increased latency in the motor swimming, adhesive removal and pole tests, as well as altered gait, foot dragging, and tremors. TUDCA administration, either before or after MPTP, significantly reduced the swimming latency, improved gait quality, and decreased foot dragging. Importantly, TUDCA was also effective in the prevention of typical parkinsonian symptoms such as spontaneous activity, ability to initiate movement and tremors. Accordingly, TUDCA prevented MPTP-induced decrease of dopaminergic fibers and ATP levels, mitochondrial dysfunction and neuroinflammation. Overall, MPTP-injected mice presented motor symptoms that are aggravated throughout time, resembling human parkinsonism, whereas PD motor symptoms were absent or mild in TUDCA-treated animals, and no aggravation was observed in any parameter. The thorough demonstration of improvement of PD symptoms together with the demonstration of the pathways triggered by TUDCA supports a subsequent clinical trial in humans and future validation of the application of this bile acid in PD.
KW - Behavioral tests
KW - MPTP
KW - Neuroinflammation
KW - Parkinson’s disease
KW - TUDCA
UR - http://www.scopus.com/inward/record.url?scp=85045271175&partnerID=8YFLogxK
U2 - 10.1007/s12035-018-1062-4
DO - 10.1007/s12035-018-1062-4
M3 - Article
C2 - 29651747
AN - SCOPUS:85045271175
SN - 0893-7648
VL - 55
SP - 9139
EP - 9155
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 12
ER -