Synthesis of Azobenzene Dyes Mediated by CotA Laccase

Ana Catarina Sousa, Sara R. Baptista, Lígia O. Martins, M. Paula Robalo

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


An eco-friendly protocol for the synthesis of azobenzene dyes by oxidative coupling of primary aromatic amines is reported. As efficient biocatalytic systems, CotA laccase and CotA laccase/ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) enable the oxidation of various substituted anilines, in aqueous medium, ambient atmosphere and under mild reaction conditions of pH and temperature. A series of azobenzene dyes were prepared in good to excellent yields in an one-pot reaction. A mechanistic proposal for the formation of the azo derivatives is presented. Our strategy offers an alternative approach for the direct synthesis of azobenzene dyes, avoiding the harsh conditions generally required for most of the traditional synthetic methods.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalChemistry - An Asian Journal
Issue number1
Publication statusPublished - 4 Jan 2019


  • biocatalysis
  • dyes/pigments
  • green chemistry
  • laccases
  • oxidative coupling


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