Synthesis, electrospinning and in vitro test of a new biodegradable gelatin-based poly(ester urethane urea) for soft tissue engineering

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9 Citations (Scopus)

Abstract

Biodegradable polyurethanes have been studied as scaffolds for tissue engineering due to their adjustable physico-chemical properties. In this work, we synthesized a biodegradable gelatin-based poly(urethane urea) using polycaprolactone-diol, as soft segment, and isophorone diisocyanate and gelatin from cold water fish skin as hard segment. The synthesis was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance and the influence of the amount of gelatin introduced in the polymer backbone was analyzed by thermal analysis. Gelatin-based poly(urethane urea) electrospun fibrous mats and solvent cast films were then produced and their physico-chemical and biological properties studied. They present an amorphous structure, elastomeric behavior and water contact angles typical of hydrophobic surfaces. Hydrolytic degradation was analyzed in phosphate buffer saline (PBS), lipase and trypsin solutions. No mass changes were detected during 37 days in PBS and trypsin while significant degradation by lipase was observed. Human foetal foreskin fibroblasts were seeded on the fibrous mats and films. Populations were evaluated by colorimetric cell viability assays and morphology by fluorescence imaging. The substrates supported cell adhesion and proliferation. The novel gelatin-based poly(urethane urea) fibrous mats offer attractive physico-chemical and biological properties for soft tissue engineering applications.

Original languageEnglish
Pages (from-to)271-281
Number of pages11
JournalEuropean Polymer Journal
Volume103
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

urethanes
tissue engineering
gelatins
Electrospinning
Gelatin
ureas
Tissue engineering
Urea
esters
Esters
Lipases
Urethane
chemical properties
Phosphates
synthesis
trypsin
Nuclear magnetic resonance
Degradation
Polycaprolactone
Lipase

Keywords

  • Biodegradable polyurethane
  • Electrospinning
  • Fish gelatin
  • In vitro tests
  • Soft tissue engineering

Cite this

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title = "Synthesis, electrospinning and in vitro test of a new biodegradable gelatin-based poly(ester urethane urea) for soft tissue engineering",
abstract = "Biodegradable polyurethanes have been studied as scaffolds for tissue engineering due to their adjustable physico-chemical properties. In this work, we synthesized a biodegradable gelatin-based poly(urethane urea) using polycaprolactone-diol, as soft segment, and isophorone diisocyanate and gelatin from cold water fish skin as hard segment. The synthesis was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance and the influence of the amount of gelatin introduced in the polymer backbone was analyzed by thermal analysis. Gelatin-based poly(urethane urea) electrospun fibrous mats and solvent cast films were then produced and their physico-chemical and biological properties studied. They present an amorphous structure, elastomeric behavior and water contact angles typical of hydrophobic surfaces. Hydrolytic degradation was analyzed in phosphate buffer saline (PBS), lipase and trypsin solutions. No mass changes were detected during 37 days in PBS and trypsin while significant degradation by lipase was observed. Human foetal foreskin fibroblasts were seeded on the fibrous mats and films. Populations were evaluated by colorimetric cell viability assays and morphology by fluorescence imaging. The substrates supported cell adhesion and proliferation. The novel gelatin-based poly(urethane urea) fibrous mats offer attractive physico-chemical and biological properties for soft tissue engineering applications.",
keywords = "Biodegradable polyurethane, Electrospinning, Fish gelatin, In vitro tests, Soft tissue engineering",
author = "T{\^a}nia Vieira and Silva, {Jorge Carvalho} and Borges, {Jo{\~a}o P.} and C{\'e}lia Henriques",
note = "Sem PDF conforme despacho. info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBD{\%}2F90682{\%}2F2012/PT# info:eu-repo/grantAgreement/FCT/5876/147333/PT# Tania Vieira acknowledges financial support from {"}Fundacao para a Ciencia e Tecnologia{"}, FCT, Portugal, through the grant SFRH/BD/90682/2012. This work was partly funded by FEDER through the COMPETE 2020 Programme and by National funds through FCT Portuguese Foundation for Science and Technology - under the project UID/CTM/50025/2013.",
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T1 - Synthesis, electrospinning and in vitro test of a new biodegradable gelatin-based poly(ester urethane urea) for soft tissue engineering

AU - Vieira, Tânia

AU - Silva, Jorge Carvalho

AU - Borges, João P.

AU - Henriques, Célia

N1 - Sem PDF conforme despacho. info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90682%2F2012/PT# info:eu-repo/grantAgreement/FCT/5876/147333/PT# Tania Vieira acknowledges financial support from "Fundacao para a Ciencia e Tecnologia", FCT, Portugal, through the grant SFRH/BD/90682/2012. This work was partly funded by FEDER through the COMPETE 2020 Programme and by National funds through FCT Portuguese Foundation for Science and Technology - under the project UID/CTM/50025/2013.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Biodegradable polyurethanes have been studied as scaffolds for tissue engineering due to their adjustable physico-chemical properties. In this work, we synthesized a biodegradable gelatin-based poly(urethane urea) using polycaprolactone-diol, as soft segment, and isophorone diisocyanate and gelatin from cold water fish skin as hard segment. The synthesis was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance and the influence of the amount of gelatin introduced in the polymer backbone was analyzed by thermal analysis. Gelatin-based poly(urethane urea) electrospun fibrous mats and solvent cast films were then produced and their physico-chemical and biological properties studied. They present an amorphous structure, elastomeric behavior and water contact angles typical of hydrophobic surfaces. Hydrolytic degradation was analyzed in phosphate buffer saline (PBS), lipase and trypsin solutions. No mass changes were detected during 37 days in PBS and trypsin while significant degradation by lipase was observed. Human foetal foreskin fibroblasts were seeded on the fibrous mats and films. Populations were evaluated by colorimetric cell viability assays and morphology by fluorescence imaging. The substrates supported cell adhesion and proliferation. The novel gelatin-based poly(urethane urea) fibrous mats offer attractive physico-chemical and biological properties for soft tissue engineering applications.

AB - Biodegradable polyurethanes have been studied as scaffolds for tissue engineering due to their adjustable physico-chemical properties. In this work, we synthesized a biodegradable gelatin-based poly(urethane urea) using polycaprolactone-diol, as soft segment, and isophorone diisocyanate and gelatin from cold water fish skin as hard segment. The synthesis was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance and the influence of the amount of gelatin introduced in the polymer backbone was analyzed by thermal analysis. Gelatin-based poly(urethane urea) electrospun fibrous mats and solvent cast films were then produced and their physico-chemical and biological properties studied. They present an amorphous structure, elastomeric behavior and water contact angles typical of hydrophobic surfaces. Hydrolytic degradation was analyzed in phosphate buffer saline (PBS), lipase and trypsin solutions. No mass changes were detected during 37 days in PBS and trypsin while significant degradation by lipase was observed. Human foetal foreskin fibroblasts were seeded on the fibrous mats and films. Populations were evaluated by colorimetric cell viability assays and morphology by fluorescence imaging. The substrates supported cell adhesion and proliferation. The novel gelatin-based poly(urethane urea) fibrous mats offer attractive physico-chemical and biological properties for soft tissue engineering applications.

KW - Biodegradable polyurethane

KW - Electrospinning

KW - Fish gelatin

KW - In vitro tests

KW - Soft tissue engineering

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U2 - 10.1016/j.eurpolymj.2018.04.005

DO - 10.1016/j.eurpolymj.2018.04.005

M3 - Article

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JO - European Polymer Journal

JF - European Polymer Journal

SN - 0014-3057

ER -