Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles

Luis Braz, Ana Grenha, Marta C. Corvo, João Paulo Lourenço, Domingos Ferreira, Bruno Sarmento, Ana M. Rosa da Costa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.

Original languageEnglish
Pages (from-to)974-985
Number of pages12
JournalCarbohydrate Polymers
Volume181
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • Carboxylated locust bean gum
  • Polyelectrolyte complexation
  • Polymeric nanoparticles
  • Sulfated locust bean gum
  • Tetraalkylammonium locust bean gum

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