TY - JOUR
T1 - Synthesis and characterization of elongated-shaped silver nanoparticles as a biocompatible anisotropic sers probe for intracellular imaging: Theoretical modeling and experimental verification
AU - Caro, Carlos
AU - Quaresma, Pedro
AU - Pereira, Eulália
AU - Franco, Jaime
AU - Leal, Manuel Pernia
AU - García-Martín, Maria Luisa
AU - Royo, Jose Luis
AU - Oliva-Montero, Jose Maria
AU - Merkling, Patrick Jacques
AU - Zaderenko, Ana Paula
AU - Pozo, David
AU - Franco, Ricardo
N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT#
info:eu-repo/grantAgreement/FCT/5876/147218/PT#
info:eu-repo/grantAgreement/FCT/3599-PPCDT/133776/PT#
This work was supported by the following grants: In Spain: P07-FQM-02595 (to CC), P10-FQM-06615 (to JMOM), P10-CTS-6928 (to DP) and PAIDI2020 Program (FQM319 to RFM and CTS677 to DP) from the Regional Ministry of Economy, Junta de Andalucia, Spain. PI-0070/2008 (to PZ) and PI-0068/2008 (to DP) from the Regional Ministry of Health, Junta de Andalucia, Spain. PI-14-1600 from the Spanish Ministry of Economy-Instituto de Salud Carlos III (to DP), and in Portugal: (a) Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO which is financed by Portuguese national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728); (b) European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundacao para a Ciencia e Tecnologia), under the Partnership Agreement PT2020 through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE), Programa Operacional Regional do Norte (ON.2 - O Novo Norte), under the Quadro de Referencia Estrategico Nacional (QREN) and funded by Fundo Europeu de Desenvolvimento Regional NORTE-01-0145-FEDER-000011; and (c) Grants EXPL/CTM-NAN/0754/2013 and PTDC/CTM-NAN/2912/2014, and post-doctoral fellowship (SFRH/BPD/84018/2012) to PQ, all financed by Fundacao para a Ciencia e a Tecnologia, Portugal.
PY - 2019/2/1
Y1 - 2019/2/1
N2 -
Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 10
7
) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe.
AB -
Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 10
7
) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe.
KW - Cancer
KW - Cell labeling
KW - Density functional theory calculations
KW - Finite element method
KW - SERS
KW - Surface enhanced Raman scattering
UR - http://www.scopus.com/inward/record.url?scp=85062560031&partnerID=8YFLogxK
U2 - 10.3390/nano9020256
DO - 10.3390/nano9020256
M3 - Article
C2 - 30781838
AN - SCOPUS:85062560031
SN - 2079-4991
VL - 9
JO - Nanomaterials
JF - Nanomaterials
IS - 2
M1 - 256
ER -