Synergy of olive bioactive phytochemicals and probiotic strain in control of Escherichia coli

C. M. Peres, Adrian Hernandez-Mendonza, M. R. Bronze, C. Peres, Xavier X. Malcata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The increasing market of probiotics from plant matrices justifies the interest on fermented olives - not only for their nutritional properties, but also because they contain health-promoting phytochemicals (such as phenolic compounds) that exhibit antibacterial, antiproliferative, anticarcinogenic and antiinflammatory properties. Additionally, olives have proven a good source and carrier of Lactobacillus strains with probiotic characteristics. However, studies focusing on the combined effect of phenolic compounds with wild probiotic bacteria have not been yet carried out. Hence, the aim of this work was to set up the combination of native olive phenolic compounds, e.g. oleuropein and hydroxytyrosol, on the recommended levels for a daily dose of olives containing probiotic bacteria - and their effect on the attachment ability and action against pathogens in situ. Tests on the health benefits of these compounds, viability and adhesion capacity of probiotic bacteria, and consequent biofilm formation, composition and relationship with adhesivity on Caco-2 model were performed. The Caco-2 cell viability results (>140%) show that there is no toxicity effect of both phenolic compounds for the levels considered (0.04 and 0.01%, w/v, for OL and HT, respectively). Conversely, these compounds can inhibit (26e50%) proliferation of human colon adenocarcinoma cells. It became clear that biofilm production on abiotic surfaces is not necessarily associated with adhesivity on biotic surfaces. The strong increase in the adhesion degree of Lactobacillus plantarum 33 after biofilm establishment prompted evaluation of the correlation between biofilm formation and adhesion capacities.

Original languageEnglish
Pages (from-to)938-945
Number of pages8
JournalLwt-Food Science And Technology
Volume64
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Probiotics
Phytochemicals
Olea
probiotics
phytopharmaceuticals
Biofilms
biofilm
Escherichia coli
phenolic compounds
adhesion
Bacteria
bacteria
oleuropein
Lactobacillus plantarum
health promotion
Caco-2 Cells
antibacterial properties
Lactobacillus
anticarcinogenic activity
Insurance Benefits

Keywords

  • Adhesion ability
  • Biofilm formation
  • Food-borne pathogens
  • Phenolic compounds
  • Potential probiotics

Cite this

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title = "Synergy of olive bioactive phytochemicals and probiotic strain in control of Escherichia coli",
abstract = "The increasing market of probiotics from plant matrices justifies the interest on fermented olives - not only for their nutritional properties, but also because they contain health-promoting phytochemicals (such as phenolic compounds) that exhibit antibacterial, antiproliferative, anticarcinogenic and antiinflammatory properties. Additionally, olives have proven a good source and carrier of Lactobacillus strains with probiotic characteristics. However, studies focusing on the combined effect of phenolic compounds with wild probiotic bacteria have not been yet carried out. Hence, the aim of this work was to set up the combination of native olive phenolic compounds, e.g. oleuropein and hydroxytyrosol, on the recommended levels for a daily dose of olives containing probiotic bacteria - and their effect on the attachment ability and action against pathogens in situ. Tests on the health benefits of these compounds, viability and adhesion capacity of probiotic bacteria, and consequent biofilm formation, composition and relationship with adhesivity on Caco-2 model were performed. The Caco-2 cell viability results (>140{\%}) show that there is no toxicity effect of both phenolic compounds for the levels considered (0.04 and 0.01{\%}, w/v, for OL and HT, respectively). Conversely, these compounds can inhibit (26e50{\%}) proliferation of human colon adenocarcinoma cells. It became clear that biofilm production on abiotic surfaces is not necessarily associated with adhesivity on biotic surfaces. The strong increase in the adhesion degree of Lactobacillus plantarum 33 after biofilm establishment prompted evaluation of the correlation between biofilm formation and adhesion capacities.",
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Synergy of olive bioactive phytochemicals and probiotic strain in control of Escherichia coli. / Peres, C. M.; Hernandez-Mendonza, Adrian; Bronze, M. R.; Peres, C.; Malcata, Xavier X.

In: Lwt-Food Science And Technology, Vol. 64, No. 2, 2015, p. 938-945.

Research output: Contribution to journalArticle

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AU - Peres, C. M.

AU - Hernandez-Mendonza, Adrian

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AU - Peres, C.

AU - Malcata, Xavier X.

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