Supercritical fluid extraction of carotenoids and chlorophylls a, b and c, from a wild strain of Scenedesmus obliquus for use in food processing

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Abstract

Faster and more selective extraction methods are urged to recover food-grade pigments and antioxidants from microalgae - which do not resort to food-incompatible and environment-unfriendly solvents. Hence, this study ascertained the influence of pressure, temperature, CO2 flow rate and a polar co-solvent upon the yields of carotenoids and chlorophylls in supercritical fluid extraction of Scenedesmus obliquus biomass. The highest carotenoid yield was attained at 250 bar and 60 degrees C. The yields of chlorophylls, when using plain CO2, increased slightly with pressure, but decreased with temperature and CO2 flow rate; the highest yield of chlorophyll a was at 4.3 g(CO2), min(-1), whereas ethanol as co-solvent increased all yields except that of chlorophyll c. The highest ratio of total carotenoids to chlorophyll a was reached at 250 bar and 60 degrees C. A remarkable selectivity was observed under these operating conditions, which may enable easy separation and purification of the aforementioned pigments. (C) 2012 Elsevier Ltd. All rights reserved.
Original languageUnknown
Pages (from-to)478-482
JournalJournal of Food Engineering
Volume116
Issue number2
DOIs
Publication statusPublished - 1 Jan 2013

Cite this

@article{dd5e9180cd2e4662afbbc4c1933bbdac,
title = "Supercritical fluid extraction of carotenoids and chlorophylls a, b and c, from a wild strain of Scenedesmus obliquus for use in food processing",
abstract = "Faster and more selective extraction methods are urged to recover food-grade pigments and antioxidants from microalgae - which do not resort to food-incompatible and environment-unfriendly solvents. Hence, this study ascertained the influence of pressure, temperature, CO2 flow rate and a polar co-solvent upon the yields of carotenoids and chlorophylls in supercritical fluid extraction of Scenedesmus obliquus biomass. The highest carotenoid yield was attained at 250 bar and 60 degrees C. The yields of chlorophylls, when using plain CO2, increased slightly with pressure, but decreased with temperature and CO2 flow rate; the highest yield of chlorophyll a was at 4.3 g(CO2), min(-1), whereas ethanol as co-solvent increased all yields except that of chlorophyll c. The highest ratio of total carotenoids to chlorophyll a was reached at 250 bar and 60 degrees C. A remarkable selectivity was observed under these operating conditions, which may enable easy separation and purification of the aforementioned pigments. (C) 2012 Elsevier Ltd. All rights reserved.",
keywords = "Food-grade ingredients, Microalgae, Antioxidants, Pigments, Supercritical fluid extraction",
author = "Duarte, {Catarina Maria} and Matias, {Ana Alexandra} and Malcata, {Francisco Xavier}",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.jfoodeng.2012.12.015",
language = "Unknown",
volume = "116",
pages = "478--482",
journal = "Journal of Food Engineering",
issn = "0260-8774",
publisher = "Elsevier Science B.V., Amsterdam.",
number = "2",

}

TY - JOUR

T1 - Supercritical fluid extraction of carotenoids and chlorophylls a, b and c, from a wild strain of Scenedesmus obliquus for use in food processing

AU - Duarte, Catarina Maria

AU - Matias, Ana Alexandra

AU - Malcata, Francisco Xavier

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Faster and more selective extraction methods are urged to recover food-grade pigments and antioxidants from microalgae - which do not resort to food-incompatible and environment-unfriendly solvents. Hence, this study ascertained the influence of pressure, temperature, CO2 flow rate and a polar co-solvent upon the yields of carotenoids and chlorophylls in supercritical fluid extraction of Scenedesmus obliquus biomass. The highest carotenoid yield was attained at 250 bar and 60 degrees C. The yields of chlorophylls, when using plain CO2, increased slightly with pressure, but decreased with temperature and CO2 flow rate; the highest yield of chlorophyll a was at 4.3 g(CO2), min(-1), whereas ethanol as co-solvent increased all yields except that of chlorophyll c. The highest ratio of total carotenoids to chlorophyll a was reached at 250 bar and 60 degrees C. A remarkable selectivity was observed under these operating conditions, which may enable easy separation and purification of the aforementioned pigments. (C) 2012 Elsevier Ltd. All rights reserved.

AB - Faster and more selective extraction methods are urged to recover food-grade pigments and antioxidants from microalgae - which do not resort to food-incompatible and environment-unfriendly solvents. Hence, this study ascertained the influence of pressure, temperature, CO2 flow rate and a polar co-solvent upon the yields of carotenoids and chlorophylls in supercritical fluid extraction of Scenedesmus obliquus biomass. The highest carotenoid yield was attained at 250 bar and 60 degrees C. The yields of chlorophylls, when using plain CO2, increased slightly with pressure, but decreased with temperature and CO2 flow rate; the highest yield of chlorophyll a was at 4.3 g(CO2), min(-1), whereas ethanol as co-solvent increased all yields except that of chlorophyll c. The highest ratio of total carotenoids to chlorophyll a was reached at 250 bar and 60 degrees C. A remarkable selectivity was observed under these operating conditions, which may enable easy separation and purification of the aforementioned pigments. (C) 2012 Elsevier Ltd. All rights reserved.

KW - Food-grade ingredients

KW - Microalgae

KW - Antioxidants

KW - Pigments

KW - Supercritical fluid extraction

U2 - 10.1016/j.jfoodeng.2012.12.015

DO - 10.1016/j.jfoodeng.2012.12.015

M3 - Article

VL - 116

SP - 478

EP - 482

JO - Journal of Food Engineering

JF - Journal of Food Engineering

SN - 0260-8774

IS - 2

ER -