Supercritical CO2 extraction of bioactive lipids from canned sardine waste streams

Liliana A. Rodrigues, Carolina V. Pereira, Ana Maria Carvalho Partidario, Luís F. Gouveia, Pedro Simoes, Alexandre Paiva, Ana A. Matias

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


In this work supercritical carbon dioxide (sc-CO2) extraction was explored to recover bioactive lipids from canned sardine residues, with special focus on triglycerides. Aiming at enhancing the recovery of the target molecules, different extraction conditions were applied, including operating pressure (300-650 bar), temperature (35-80 °C) and CO2 flow rate (5-25 g/min). The performance of sc-CO2 extractions was compared with a conventional Bligh and Dyer extraction, and the resulting samples were characterized in terms of global yield and fatty acid profile. To evaluate their potential application as bioactive ingredients, extracts were screened for cytotoxicity, oxidative damage, and antiproliferative and anti-inflammatory activities in human intestinal cells (Caco-2 and HT-29). Our results showed that sc-CO2 extraction was able to produce extracts with antiproliferative and anti-inflammatory properties, which can be potentially used as ingredients in different industrial health-related applications, namely dietary/nutritional supplements, or pharmaceutical products.

Original languageEnglish
JournalJournal of CO2 Utilization
Publication statusPublished - 2021


  • Anti-inflammatory activity
  • Antiproliferative activity
  • Bioactive fatty acids
  • Canned sardine residues
  • Supercritical CO extraction


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