TY - JOUR
T1 - Supercritical CO2 and subcritical water technologies for the production of bioactive extracts from sardine (Sardina pilchardus) waste
AU - Melgosa, Rodrigo
AU - Trigueros, Esther
AU - Sanz, María Teresa
AU - Cardeira, Martim
AU - Rodrigues, Liliana A.
AU - Fernández, Naiara
AU - Matias, Ana A.
AU - Bronze, Maria Rosário
AU - Marques, Marta
AU - Paiva, Alexandre
AU - Simões, Pedro
N1 - info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FASP-PES%2F28399%2F2017/PT#
info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01146%2F2015%2FCP1293%2FCT0003/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F116002%2F2016/PT#
This work was supported by the Associate Laboratory for Green Chemistry-LAQV which is financed by national funds from FCT/MCTES (UIDB/QUI/50006/2020).
We acknowledge the help of Nuno Costa and Carla Rodrigues from Laboratorio de Analises (LAQV, REQUIMTE). Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - The valorization of sardine (Sardina pilchardus) waste (SW) from a canning facility has been investigated within a biorefining approach. Sequential fractionation of SW into its constituents has been carried out using green solvents such as supercritical carbon dioxide (SCCO2) and subcritical water (sCW). The lipid fraction has been isolated through supercritical fluid extraction (SFE) with SCCO2 at 250 bar and 40 °C, yielding 20.3 ± 0.2 g oil/100 g SW with up to 17.2 %wt. omega-3 polyunsaturated fatty acids (PUFAs). Aiming at the protein fraction, sCW extraction/hydrolysis has been carried out at different temperatures (90, 140, 190 and 250 °C), using both SW and defatted sardine waste (DSW) from SFE experiments. Previous defatting increased protein recovery and purity. Bioactive properties of the fish protein hydrolysates (FPHs) obtained were affected by the extraction temperature. The highest antioxidant activity and in vitro antiproliferative effect were found in the extracts obtained at 250 °C.
AB - The valorization of sardine (Sardina pilchardus) waste (SW) from a canning facility has been investigated within a biorefining approach. Sequential fractionation of SW into its constituents has been carried out using green solvents such as supercritical carbon dioxide (SCCO2) and subcritical water (sCW). The lipid fraction has been isolated through supercritical fluid extraction (SFE) with SCCO2 at 250 bar and 40 °C, yielding 20.3 ± 0.2 g oil/100 g SW with up to 17.2 %wt. omega-3 polyunsaturated fatty acids (PUFAs). Aiming at the protein fraction, sCW extraction/hydrolysis has been carried out at different temperatures (90, 140, 190 and 250 °C), using both SW and defatted sardine waste (DSW) from SFE experiments. Previous defatting increased protein recovery and purity. Bioactive properties of the fish protein hydrolysates (FPHs) obtained were affected by the extraction temperature. The highest antioxidant activity and in vitro antiproliferative effect were found in the extracts obtained at 250 °C.
KW - Antioxidant activity
KW - Antiproliferative activity
KW - Biorefinery
KW - Fish protein hydrolysate
KW - Sardine waste
KW - Subcritical water
UR - http://www.scopus.com/inward/record.url?scp=85086784821&partnerID=8YFLogxK
U2 - 10.1016/j.supflu.2020.104943
DO - 10.1016/j.supflu.2020.104943
M3 - Article
AN - SCOPUS:85086784821
SN - 0896-8446
VL - 164
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
M1 - 104943
ER -