TY - JOUR
T1 - Supercritical CO2 assisted impregnation of ibuprofen on medium-chain-length polyhydroxyalkanoates (mcl-PHA)
AU - Meneses, Liane
AU - Craveiro, Rita
AU - Jesus, Ana Rita
AU - Reis, Maria A. M.
AU - Freitas, Filomena
AU - Paiva, Alexandre
N1 - This work is financed by national funds from FCT-Fundacao para a Ciencia e a Tecnologia, I.P., in the scope of the project UIDB/50006/2020 of the Associate Laboratory for Green Chemistry-LAQV, the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences-UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. Liane Meneses and Alexandre Paiva would like to acknowledge FCT for the financial support through SFRH/BD/148510/2019 and IF/01146/2015.
PY - 2021/8/6
Y1 - 2021/8/6
N2 - In this work, we propose the utilization of scCO2 to impregnate ibuprofen into the mcl- PHA matrix produced by Pseudomonas chlororaphis subs. aurantiaca (DSM 19603). The biopolymer has adhesive properties, is biocompatible and has a melting temperature of 45 °C. Several conditions, namely, pressure (15 and 20 MPa) and impregnation time (30 min, 1 h and 3 h) were tested. The highest ibuprofen content (90.8 ± 6.5 mg of ibuprofen/gPHA) was obtained at 20 MPa and 40 °C, for 1 h, with an impregnation rate of 89 mg/(g.h). The processed mcl-PHA samples suffered a plasticization, as shown by the decrease of 6.5 °C in the Tg, at 20 MPa. The polymer’s crystallinity was also affected concomitantly with the matrices’ ibuprofen content. For all the impregnation conditions tested the release of ibuprofen from the biopolymer followed a type II release profile. This study has demonstrated that the mcl-PHA produced by P. chlororaphis has a great potential for the development of novel topical drug delivery systems.
AB - In this work, we propose the utilization of scCO2 to impregnate ibuprofen into the mcl- PHA matrix produced by Pseudomonas chlororaphis subs. aurantiaca (DSM 19603). The biopolymer has adhesive properties, is biocompatible and has a melting temperature of 45 °C. Several conditions, namely, pressure (15 and 20 MPa) and impregnation time (30 min, 1 h and 3 h) were tested. The highest ibuprofen content (90.8 ± 6.5 mg of ibuprofen/gPHA) was obtained at 20 MPa and 40 °C, for 1 h, with an impregnation rate of 89 mg/(g.h). The processed mcl-PHA samples suffered a plasticization, as shown by the decrease of 6.5 °C in the Tg, at 20 MPa. The polymer’s crystallinity was also affected concomitantly with the matrices’ ibuprofen content. For all the impregnation conditions tested the release of ibuprofen from the biopolymer followed a type II release profile. This study has demonstrated that the mcl-PHA produced by P. chlororaphis has a great potential for the development of novel topical drug delivery systems.
KW - Alternative technologies
KW - Controlled drug release
KW - Green solvents
KW - Ibuprofen
KW - Impregnation
KW - Medium-chain-length polyhydroxyalkanoates
KW - Supercritical carbon dioxide
UR - http://www.scopus.com/inward/record.url?scp=85112278465&partnerID=8YFLogxK
U2 - 10.3390/molecules26164772
DO - 10.3390/molecules26164772
M3 - Article
C2 - 34443357
AN - SCOPUS:85112278465
VL - 26
JO - Molecules (Basel, Switzerland)
JF - Molecules (Basel, Switzerland)
SN - 1420-3049
IS - 16
M1 - 4772
ER -