Super-beacons: Open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy

Pedro Matos Pereira, Nils Gustafsson, Mark Marsh, Musa M. Mhlanga, Ricardo Henriques

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Localization-based super-resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non-fluorescent states. These transitions are commonly regulated by high-intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single-molecule self-quenching as a mechanism to generate spontaneous photoswitching. To demonstrate this principle, we developed a new class of DNA-based open-source super-resolution probes named super-beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live-cell compatible super-resolution microscopy without high-illumination or toxic imaging buffers is revealed by imaging interferon inducible transmembrane proteins (IFITMs) at sub-100 nm resolutions.

Original languageEnglish
Pages (from-to)375-385
Number of pages11
JournalTraffic
Volume21
Issue number5
DOIs
Publication statusPublished - 1 May 2020

Keywords

  • DNA
  • fluorescence microscopy
  • fluorescence quenching
  • live-cell
  • molecular-beacons
  • super-resolution

Fingerprint

Dive into the research topics of 'Super-beacons: Open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy'. Together they form a unique fingerprint.

Cite this