Succinic acid production from glycerol by Actinobacillus succinogenes using dimethylsulfoxide as electron acceptor

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Glycerol, a highly abundant byproduct of the biodiesel industry, constitutes today a cheap feedstock for biobased succinic acid (SA) production. Actinobacillus succinogenes is one of the best SA producers. However, glycerol consumption by this biocatalyst is limited because of a redox imbalance during cell growth. The use of an external electron acceptor may improve the metabolism of SA synthesis by A. succinogenes in glycerol. In this study, the effect of dimethylsulfoxide (DMSO), an electron acceptor, on glycerol consumption and SA production by A. succinogenes under controlled fermentation conditions was investigated. Concentrations of DMSO between 1 and 4% (v/v) greatly promoted glycerol consumption and SA production by A. succinogenes. During fed-batch cultivation, SA concentration reached 49.62 g/L, with a product yield of 0.87 gSA/gGLR and a maximum production rate of 2.31 gSA/Lh, the highest values so far reported in the literature for A. succinogenes using glycerol as carbon source. These results show that using DMSO as external electron acceptor significantly promotes glycerol consumption and succinic acid production by A. succinogenes and may be used as a co-substrate, opening new perspectives for the use of glycerol by this biocatalyst.
Original languageEnglish
Pages (from-to)133-139
JournalNew Biotechnology
Volume31
Issue number1
DOIs
Publication statusPublished - 25 Jan 2014

Keywords

  • MANNHEIMIA-SUCCINICIPRODUCENS
  • BOVINE RUMEN
  • FERMENTATION
  • BACTERIUM
  • KINETICS
  • STRAIN

Fingerprint Dive into the research topics of 'Succinic acid production from glycerol by Actinobacillus succinogenes using dimethylsulfoxide as electron acceptor'. Together they form a unique fingerprint.

Cite this