Subproteomic signature comparison of in vitro selected fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104B

Susana Correia, Michel Hébraud, Ingrid Chafsey, Christophe Chambon, Didier Viala, Carmen Torres, Manuela Caniça, José Luis Capelo, Patrícia Poeta, Gilberto Igrejas

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strain (Se6), and also in Se6-M under ciprofloxacin (CIP) stress, were evaluated in order to give new insights into the mechanisms involved. Proteomes were compared at the intracellular and membrane levels by a 2-DE~LC-MS/MS and a shotgun LC-MS/MS approach, respectively. Results: In total, 35 differentially abundant proteins were identified when comparing Se6 with Se6-M (25 more abundant in Se6 and 10 more abundant in Se6-M) and 82 were identified between Se6-M and Se6-M+CIP (51 more abundant in Se6-M and 31 more abundant under ciprofloxacin stress). Conclusion: Several proteins with known and possible roles in quinolone resistance were identified which provide important information about mechanism-related differential protein expression, supporting the current knowledge and also leading to new testable hypotheses on the mechanism of action of fluoroquinolone drugs.

Original languageEnglish
Pages (from-to)941-961
Number of pages21
JournalExpert Review of Proteomics
Volume14
Issue number10
DOIs
Publication statusPublished - 3 Oct 2017

Keywords

  • 2-DE
  • Antimicrobial resistance
  • comparative subproteomics
  • LC-MS/MS
  • Salmonella Typhimurium DT104B
  • shotgun proteomics

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