Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal

Margarida Alves, Lihua Xiao, Irshad Sulaiman, Altaf A. Lal, Olga Matos, Francisco Antunes

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347 Citations (Scopus)

Abstract

Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.

Original languageEnglish
Pages (from-to)2744-2747
Number of pages4
JournalJournal Of Clinical Microbiology
Volume41
Issue number6
DOIs
Publication statusPublished - 1 Jun 2003

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