Study of a Cold Atmospheric Pressure Plasma jet device for indirect treatment of Squamous Cell Carcinoma

The possibility of obtaining plasmas at low temperatures and atmospheric conditions, the Cold Atmospheric Plasmas (CAPs), has triggered several studies on its possible medical applications. The use of CAPs devices applied to oncological treatments is still in a pre-clinical stage, but early investigations have shown promising results, both in vitro and in vivo. In this work, is reported the study of a custom made Cold Atmospheric Pressure Plasma jet device for indirect treatment of Squamous Cell Carcinoma. The plasma plume produced by the jet device was characterized by optical emission spectroscopy and the influence of different working parameters such as plasma exposure time, the number of cells/mL and the volume of medium used for the treatments, were systematically tested and studied. According to the obtained results, it can be further observed that the in vitro anti-cancer capacity of the developed jet plasma device will be higher when small volumes of medium are used together with moderate plasma treatment times, keeping the non-cancerous cells almost unaffected.