Studies on antimalarial drug susceptibility in Colombia, in relation to Pfmdr1 and Pfcrt

E Restrepo-Pineda, E Arango, A Maestre, V E Do Rosário, P Cravo

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

In Colombia, Plasmodium resistance to antimalarials such as chloroquine and antifolates is a serious problem. As a result, the national Colombian health authorities are monitoring the efficacy of alternative drugs and schemes. The study of genetic polymorphisms related with drug resistance is required in the region. In vitro responses to chloroquine, quinine, mefloquine, amodiaquine, desethylamodiaquine, artesunate and dihydroartesunate were carried out by HRP ELISA. SNP analysis in Pfcrt and Pfmdr1 genes was performed by PCR-RFLP in 77 samples from the North West region of Colombia. In vitro resistance to chloroquine was high (74%), followed by mefloquine (30%) and desethylamodiaquine (30%). A positive correlation between the IC(50) of paired drugs was also detected. The allele Pfmdr1 N86 (wild) was present in 100% of the samples and 1246Y (mutant) in 92%. However, their presence did not correlate with in vitro drug resistance. Presence of the mutations K76T and N75E in Pfcrt was confirmed in all samples. Analysis of 4 codons (72, 74, 75 and 76) in pfcrt confirmed the presence of the haplotypes CMET in 91% and SMET in 9% of the samples.

Original languageEnglish
Pages (from-to)547-553
Number of pages6
JournalParasitology
Volume135
Issue number5
DOIs
Publication statusPublished - May 2008

Keywords

  • Amodiaquine
  • Animals
  • Antimalarials
  • Chloroquine
  • Colombia
  • Drug Resistance
  • Humans
  • Malaria, Falciparum
  • Mefloquine
  • Membrane Transport Proteins
  • Multidrug Resistance-Associated Proteins
  • Parasitic Sensitivity Tests
  • Plasmodium falciparum
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide
  • Protozoan Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

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