Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon-gamma and interleukin-4 mRNA expression

Cláudia S Marques; Nuno Rolão; Sónia Centeno-Lima; Hélder Lousada; Carla Maia; Lenea Campino; Virgilio Estólio do Rosário; Henrique Silveira

doi:10.1590/s0074-02762005000800011

Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon-gamma and interleukin-4 mRNA expression

Cláudia S Marques, Nuno Rolão, Sónia Centeno-Lima, Hélder Lousada, Carla Maia, Lenea Campino, Virgilio Estólio do Rosário, Henrique Silveira

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10 Citations (Scopus)

Abstract

This work aimed to study the T helper type 1/2 (Th1/Th2) cytokine profile in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum. Expression of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was analyzed, in spleen and liver of C57BL/6 mice, by reverse transcriptase-polymerase chain reaction. High levels of IFN-gamma expression did not prevent the progression of Leishmania in co-infected mice and Leishmania infection did not interfere with the Th1/Th2 switch necessary for Plasmodium control. The presence of IL-4 at day 28 in co-infected mice, essential for Plasmodium elimination, was probably a key factor on the exacerbation of the Leishmania infection.

Original language	English
Pages (from-to)	889-892
Number of pages	4
Journal	Memórias do Instituto Oswaldo Cruz
Volume	100
Issue number	8
DOIs	https://doi.org/10.1590/s0074-02762005000800011
Publication status	Published - Dec 2005

Keywords

Animals
Disease Models, Animal
Female
Interferon-gamma
Interleukin-4
Leishmania infantum
Leishmaniasis, Visceral
Liver
Malaria
Mice
Mice, Inbred C57BL
Parasitemia
Plasmodium chabaudi
RNA, Messenger
RNA, Protozoan
Reverse Transcriptase Polymerase Chain Reaction
Spleen
Th1 Cells
Th2 Cells
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1590/s0074-02762005000800011

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Marques, C. S., Rolão, N., Centeno-Lima, S., Lousada, H., Maia, C., Campino, L., Rosário, V. E. D., & Silveira, H. (2005). Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon-gamma and interleukin-4 mRNA expression. Memórias do Instituto Oswaldo Cruz, 100(8), 889-892. https://doi.org/10.1590/s0074-02762005000800011

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abstract = "This work aimed to study the T helper type 1/2 (Th1/Th2) cytokine profile in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum. Expression of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was analyzed, in spleen and liver of C57BL/6 mice, by reverse transcriptase-polymerase chain reaction. High levels of IFN-gamma expression did not prevent the progression of Leishmania in co-infected mice and Leishmania infection did not interfere with the Th1/Th2 switch necessary for Plasmodium control. The presence of IL-4 at day 28 in co-infected mice, essential for Plasmodium elimination, was probably a key factor on the exacerbation of the Leishmania infection.",

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Marques, CS, Rolão, N , Centeno-Lima, S, Lousada, H, Maia, C , Campino, L , Rosário, VED & Silveira, H 2005, 'Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon-gamma and interleukin-4 mRNA expression', Memórias do Instituto Oswaldo Cruz, vol. 100, no. 8, pp. 889-892. https://doi.org/10.1590/s0074-02762005000800011

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T2 - interferon-gamma and interleukin-4 mRNA expression

AU - Marques, Cláudia S

AU - Rolão, Nuno

AU - Centeno-Lima, Sónia

AU - Lousada, Hélder

AU - Maia, Carla

AU - Campino, Lenea

AU - Rosário, Virgilio Estólio do

AU - Silveira, Henrique

PY - 2005/12

Y1 - 2005/12

N2 - This work aimed to study the T helper type 1/2 (Th1/Th2) cytokine profile in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum. Expression of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was analyzed, in spleen and liver of C57BL/6 mice, by reverse transcriptase-polymerase chain reaction. High levels of IFN-gamma expression did not prevent the progression of Leishmania in co-infected mice and Leishmania infection did not interfere with the Th1/Th2 switch necessary for Plasmodium control. The presence of IL-4 at day 28 in co-infected mice, essential for Plasmodium elimination, was probably a key factor on the exacerbation of the Leishmania infection.

AB - This work aimed to study the T helper type 1/2 (Th1/Th2) cytokine profile in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum. Expression of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was analyzed, in spleen and liver of C57BL/6 mice, by reverse transcriptase-polymerase chain reaction. High levels of IFN-gamma expression did not prevent the progression of Leishmania in co-infected mice and Leishmania infection did not interfere with the Th1/Th2 switch necessary for Plasmodium control. The presence of IL-4 at day 28 in co-infected mice, essential for Plasmodium elimination, was probably a key factor on the exacerbation of the Leishmania infection.

KW - Animals

KW - Disease Models, Animal

KW - Female

KW - Interferon-gamma

KW - Interleukin-4

KW - Leishmania infantum

KW - Leishmaniasis, Visceral

KW - Liver

KW - Malaria

KW - Mice

KW - Mice, Inbred C57BL

KW - Parasitemia

KW - Plasmodium chabaudi

KW - RNA, Messenger

KW - RNA, Protozoan

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Spleen

KW - Th1 Cells

KW - Th2 Cells

KW - Journal Article

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UR - https://pubmed.ncbi.nlm.nih.gov/16444421/

U2 - 10.1590/s0074-02762005000800011

DO - 10.1590/s0074-02762005000800011

M3 - Article

C2 - 16444421

SN - 0074-0276

VL - 100

SP - 889

EP - 892

JO - Memórias do Instituto Oswaldo Cruz

JF - Memórias do Instituto Oswaldo Cruz

IS - 8

ER -

Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon-gamma and interleukin-4 mRNA expression

Abstract

Keywords

UN SDGs

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