Structure of a dioxygen reduction enzyme from Desulfovibrio gigas

Carlos Frazão; Gabriela G. F. Silva; Cláudio M. Gomes; Pedro Matias; Ricardo Coelho; Larry Sieker; Sofia Macedo; Ming Y. Liu; Solange Oliveira; Miguel Teixeira; António V. Xavier; Claudina Rodrigues-Pousada; Maria A. Carrondo; Jean Le Gall

doi:10.1038/80961

Structure of a dioxygen reduction enzyme from Desulfovibrio gigas

Carlos Frazão, Gabriela G. F. Silva, Cláudio M. Gomes, Pedro Matias, Ricardo Coelho, Larry Sieker, Sofia Macedo, Ming Y. Liu, Solange Oliveira, Miguel Teixeira, António V. Xavier, Claudina Rodrigues-Pousada, Maria A. Carrondo, Jean Le Gall

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin: Oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 ∅ resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-β-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.

Original language	English
Pages (from-to)	1041-1045
Number of pages	5
Journal	Nature Structural Biology
Volume	7
Issue number	11
DOIs	https://doi.org/10.1038/80961
Publication status	Published - 18 Nov 2000

Keywords

oxygen oxidoreductase
water
beta lactamase
flavodoxin
iron
monomer
oxidoreductase
unclassified drug
rubredoxin
oxygen

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abstract = "Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin: Oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 ∅ resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-β-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.",

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AU - Frazão, Carlos

AU - Silva, Gabriela G. F.

AU - Gomes, Cláudio M.

AU - Matias, Pedro

AU - Coelho, Ricardo

AU - Sieker, Larry

AU - Macedo, Sofia

AU - Liu, Ming Y.

AU - Oliveira, Solange

AU - Teixeira, Miguel

AU - Xavier, António V.

AU - Rodrigues-Pousada, Claudina

AU - Carrondo, Maria A.

AU - Le Gall, Jean

PY - 2000/11/18

Y1 - 2000/11/18

N2 - Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin: Oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 ∅ resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-β-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.

AB - Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin: Oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 ∅ resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-β-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.

KW - oxygen oxidoreductase

KW - water

KW - beta lactamase

KW - flavodoxin

KW - iron

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KW - oxidoreductase

KW - unclassified drug

KW - rubredoxin

KW - oxygen

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JF - Nature Structural Biology

IS - 11

ER -

Structure of a dioxygen reduction enzyme from Desulfovibrio gigas

Abstract

Keywords

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