Structure and function of ferrochelatase

Gloria C. Ferreira, Ricardo Franco, Steven G. Lloyd, Isabel Moura, José J. G. Moura, Boi H. Huynh

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Ferrochelatase is the terminal enzyme of the heme biosynthetic pathway in all cells. It catalyzes the insertion of ferrous iron into protoporphyrin IX, yielding heme. In eukaryotic cells, ferrochelatase is a mitochondrial inner membrane-associated protein with the active site facing the matrix. Decreased values of ferrochelatase activity in all tissues are a characteristic of patients with protoporphyria. Point-mutations in the ferrochelatase gene have been recently found to be associated with certain cases of erythropoietic protoporphyria. During the past four years, there have been considerable advances in different aspects related to structure and function of ferrochelatase. Genomic and cDNA clones for bacteria, yeast, barley, mouse, and human ferrochelatase have been isolated and sequenced. Functional expression of yeast ferrochelatase in yeast strains deficient in this enzyme, and expression in Escherichia coli and in baculovirusinfected insect cells of different ferrochelatase cDNAs have been accomplished. A recently identified (2Fe-2S) cluster appears to be a structural feature shared among mammalian ferrochelatases. Finally, functional studies of ferrochelatase site-directed mutants, in which key amino acids were replaced with residues identified in some cases of protoporphyria, will be summarized in the context of protein structure.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalJournal Of Bioenergetics And Biomembranes
Volume27
Issue number2
DOIs
Publication statusPublished - 1 Apr 1995

Fingerprint

Ferrochelatase
Yeasts
Heme
Complementary DNA
Erythropoietic Protoporphyria
Biosynthetic Pathways
Eukaryotic Cells
Enzymes
Hordeum
Point Mutation
Insects
Catalytic Domain
Membrane Proteins
Clone Cells

Keywords

  • Heme
  • heme metabolism
  • iron-sulfur cluster
  • mitochondria
  • porphyrin

Cite this

Ferreira, Gloria C. ; Franco, Ricardo ; Lloyd, Steven G. ; Moura, Isabel ; Moura, José J. G. ; Huynh, Boi H. / Structure and function of ferrochelatase. In: Journal Of Bioenergetics And Biomembranes. 1995 ; Vol. 27, No. 2. pp. 221-229.
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Structure and function of ferrochelatase. / Ferreira, Gloria C.; Franco, Ricardo; Lloyd, Steven G.; Moura, Isabel; Moura, José J. G.; Huynh, Boi H.

In: Journal Of Bioenergetics And Biomembranes, Vol. 27, No. 2, 01.04.1995, p. 221-229.

Research output: Contribution to journalArticle

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