@article{b58291f70c5a4126bb82bbfd1de69498,
title = "Structural Insights into the Intrinsically Disordered GPCR C-Terminal Region, Major Actor in Arrestin-GPCR Interaction",
abstract = "Arrestin-dependent pathways are a central component of G protein-coupled receptor (GPCRs) signaling. However, the molecular processes regulating arrestin binding are to be further illuminated, in particular with regard to the structural impact of GPCR C-terminal disordered regions. Here, we used an integrated biophysical strategy to describe the basal conformations of the C-terminal domains of three class A GPCRs, the vasopressin V2 receptor (V2R), the growth hormone secretagogue or ghrelin receptor type 1a (GHSR) and the β2-adernergic receptor (β2AR). By doing so, we revealed the presence of transient secondary structures in these regions that are potentially involved in the interaction with arrestin. These secondary structure elements differ from those described in the literature in interaction with arrestin. This suggests a mechanism where the secondary structure conformational preferences in the C-terminal regions of GPCRs could be a central feature for optimizing arrestins recognition.",
keywords = "arrestin, GPCR, intrinsically disordered proteins or regions (IDPs/IDRs), NMR",
author = "Myriam Guillien and Assia Mouhand and Aur{\'e}lie Fournet and Amandine Gontier and Navia, {Aleix Mart{\'i}} and Cordeiro, {Tiago N.} and Fr{\'e}d{\'e}ric Allemand and Aur{\'e}lien Thureau and Ban{\`e}res, {Jean Louis} and Pau Bernad{\'o} and Nathalie Sibille",
note = "Funding Information: Funding: This work has benefited from support by the Labex EpiGenMed, an «Investissements d{\textquoteright}avenir» program, reference ANR-10-LABX-12-01 awarded to M.G. N.S. and J.L.B. acknowledge the support of the ANR GPCteR (ANR-17-CE11-0022-01). The CBS is a member of the French Infrastructure for Integrated Structural Biology (FRISBI) supported by the French National Research Agency (ANR-10-INSB-05). Funding Information: This work has benefited from support by the Labex EpiGenMed, an ?Investissements d?avenir? program, reference ANR-10-LABX-12-01 awarded to M.G. N.S. and J.L.B. acknowledge the support of the ANR GPCteR (ANR-17-CE11-0022-01). The CBS is a member of the French Infrastructure for Integrated Structural Biology (FRISBI) supported by the French National Research Agency (ANR-10-INSB-05). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = may,
doi = "10.3390/biom12050617",
language = "English",
volume = "12",
journal = "Biomolecules",
issn = "2218-273X",
publisher = "MDPI - Multidisciplinary Digital Publishing Institute",
number = "5",
}