Structural features of a DPPG liposome layer adsorbed on a rough surface

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of drug delivery systems, sensors and other devices based on liposomes (small unilamellar lipid vesicles, SUVs) requires the adsorption of intact lipid structures onto solid surfaces in the first place. In this work, we report on the in situ investigation of the adsorption of liposomes of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DPPG) onto a rough surface by neutron reflectivity. Rough surfaces are achieved by preparing polyelectrolyte layer-by-layer films, which act as soft polymer cushions. Neutron reflectivity measurements performed at the solid/D 2 O interface allow for the determination of the thickness of the adsorbed structures. The conducted investigation proofs that the liposomes dispersed in the liquid phase are generally adsorbed intact onto the cushion surface, confirming that the roughness of the latter is a variable to be taken into account if one intends to adsorb intact lipid structures. Liposome flattening is observed and justified by the attractive electrostatic interactions occurring between the negatively charged lipid liposomes and the outermost, positively charged polyelectrolyte layer of the cushion. The conducted measurements further demonstrate that the adsorbed liposomes are stable for several hours. These findings are fundamental for the development of devices based on immobilized but intact SUVs on sensor surfaces.

Original languageEnglish
Title of host publicationComputational Intelligence Methods for Bioinformatics and Biostatistics - 14th International Meeting, CIBB 2017, Revised Selected Papers
EditorsAlberto Policriti, Roberto Tagliaferri, Massimo Bartoletti, Andrea Bracciali, Annalisa Barla, Gunnar W. Klau, Leif Peterson
PublisherSpringer Verlag
Pages138-144
Number of pages7
ISBN (Print)9783030141592
DOIs
Publication statusPublished - 1 Jan 2019
Event14th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2017 - Cagliari, Italy
Duration: 7 Sep 20179 Sep 2017

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
PublisherSpringer Verlag
Volume10834 LNBI
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference14th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2017
CountryItaly
CityCagliari
Period7/09/179/09/17

Keywords

  • Adsorption
  • Liposome
  • Neutron reflectivity
  • Roughness
  • Surface

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