TY - JOUR
T1 - Structural and functional insights into the activation of the dual incision activity of UvrC, a key player in bacterial NER
AU - Seck, Anna
AU - De Bonis, Salvatore
AU - Stelter, Meike
AU - Ökvist, Mats
AU - Senarisoy, Müge
AU - Hayek, Mohammad Rida
AU - Le Roy, Aline
AU - Martin, Lydie
AU - Saint-Pierre, Christine
AU - Silveira, Célia M.
AU - Gasparutto, Didier
AU - Todorovic, Smilja
AU - Ravanat, Jean Luc
AU - Timmins, Joanna
N1 - Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.
PY - 2023/4/11
Y1 - 2023/4/11
N2 - Bacterial nucleotide excision repair (NER), mediated by the UvrA, UvrB and UvrC proteins is a multistep, ATP-dependent process, that is responsible for the removal of a very wide range of chemically and structurally diverse DNA lesions. DNA damage removal is performed by UvrC, an enzyme possessing a dual endonuclease activity, capable of incising the DNA on either side of the damaged site to release a short single-stranded DNA fragment containing the lesion. Using biochemical and biophysical approaches, we have probed the oligomeric state, UvrB-and DNA-binding abilities and incision activities of wild-Type and mutant constructs of UvrC from the radiation resistant bacterium, Deinococcus radiodurans. Moreover, by combining the power of new structure prediction algorithms and experimental crystallographic data, we have assembled the first model of a complete UvrC, revealing several unexpected structural motifs and in particular, a central inactive RNase H domain acting as a platform for the surrounding domains. In this configuration, UvrC is maintained in a 'closed' inactive state that needs to undergo a major rearrangement to adopt an 'open' active state capable of performing the dual incision reaction. Taken together, this study provides important insight into the mechanism of recruitment and activation of UvrC during NER.
AB - Bacterial nucleotide excision repair (NER), mediated by the UvrA, UvrB and UvrC proteins is a multistep, ATP-dependent process, that is responsible for the removal of a very wide range of chemically and structurally diverse DNA lesions. DNA damage removal is performed by UvrC, an enzyme possessing a dual endonuclease activity, capable of incising the DNA on either side of the damaged site to release a short single-stranded DNA fragment containing the lesion. Using biochemical and biophysical approaches, we have probed the oligomeric state, UvrB-and DNA-binding abilities and incision activities of wild-Type and mutant constructs of UvrC from the radiation resistant bacterium, Deinococcus radiodurans. Moreover, by combining the power of new structure prediction algorithms and experimental crystallographic data, we have assembled the first model of a complete UvrC, revealing several unexpected structural motifs and in particular, a central inactive RNase H domain acting as a platform for the surrounding domains. In this configuration, UvrC is maintained in a 'closed' inactive state that needs to undergo a major rearrangement to adopt an 'open' active state capable of performing the dual incision reaction. Taken together, this study provides important insight into the mechanism of recruitment and activation of UvrC during NER.
UR - http://www.scopus.com/inward/record.url?scp=85152244641&partnerID=8YFLogxK
U2 - 10.1093/nar/gkad108
DO - 10.1093/nar/gkad108
M3 - Article
C2 - 36869664
AN - SCOPUS:85152244641
SN - 0305-1048
VL - 51
SP - 2931
EP - 2949
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 6
ER -