TY - JOUR
T1 - Structural and functional analysis of the gene cluster encoding carotenoid biosynthesis in Mycobacterium aurum A+
AU - Viveiros, Miguel
AU - Krubasik, Philipp
AU - Sandmann, Gerhard
AU - Houssaini-Iraqui, Mohamed
PY - 2000/6/1
Y1 - 2000/6/1
N2 - The fragment containing the carotenoid gene cluster from Mycobacterium aurum A+, a 3,3'-dihydroxy-isoneriatene and 3-monohydroxy-isoneriatene accumulator, has been sequenced and the exposed eight genes are organised in two operons. The function of three of these genes, a phytoene desaturase (crtI), a phytoene cyclase (crtY) and a β-carotene desaturase (crtU), was demonstrated by complementation of M. aurum carotenoid mutants. The eight genes of the carotenoid cluster are highly homologous to other carotenoid gene clusters and thus this cluster is a candidate for its introduction into mycobacteria as a non-antibiotic reporter gene(s) as well as a source of new regulated promoters. Copyright (C) 2000 Federation of European Microbiological Societies.
AB - The fragment containing the carotenoid gene cluster from Mycobacterium aurum A+, a 3,3'-dihydroxy-isoneriatene and 3-monohydroxy-isoneriatene accumulator, has been sequenced and the exposed eight genes are organised in two operons. The function of three of these genes, a phytoene desaturase (crtI), a phytoene cyclase (crtY) and a β-carotene desaturase (crtU), was demonstrated by complementation of M. aurum carotenoid mutants. The eight genes of the carotenoid cluster are highly homologous to other carotenoid gene clusters and thus this cluster is a candidate for its introduction into mycobacteria as a non-antibiotic reporter gene(s) as well as a source of new regulated promoters. Copyright (C) 2000 Federation of European Microbiological Societies.
KW - Carotenogenic gene cluster expression
KW - Isoneriatene synthase
KW - Mycobacterium aurum
KW - Phytoene cyclase
KW - Phytoene desaturase
UR - http://www.scopus.com/inward/record.url?scp=0034213794&partnerID=8YFLogxK
UR - https://academic.oup.com/femsle/article/187/1/95/474842
U2 - 10.1016/S0378-1097(00)00182-8
DO - 10.1016/S0378-1097(00)00182-8
M3 - Article
C2 - 10828407
AN - SCOPUS:0034213794
SN - 0378-1097
VL - Vol. 187
SP - 95
EP - 101
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - n.º 1
ER -
