TY - JOUR
T1 - Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides
AU - Pandeirada, Carolina O.
AU - Maricato, Élia
AU - Ferreira, Sónia S.
AU - Correia, Viviana G.
AU - Pinheiro, Benedita A.
AU - Evtuguin, Dmitry V.
AU - Palma, Angelina S.
AU - Correia, Alexandra
AU - Vilanova, Manuel
AU - Coimbra, Manuel A.
AU - Nunes, Cláudia
N1 - info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91623%2F2012/PT#
info:eu-repo/grantAgreement/FCT/PD/PD%2FBD%2F105727%2F2014/PT#
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87245%2F2012/PT#
This work was developed within the scope of CICECO-Aveiro Institute of Materials (FCT UID/CTM/50011/2019).
QOPNA (FCT UID/QUI/00062/2019).
Applied Molecular Biosciences UnitUCIBIO (UID/Multi/04378/2019), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement. CN, EM, SSF, VC, ASP, and AC thank FCT for the individual grants (SFRH/BPD/100627/2014, SFRH/BD/103003/2014, IF/00033/2012. We are grateful to Ten Feizi and Lisete Silva from the Glycosciences Laboratory (Imperial College London, UK) for their support and assistance on robotic microarray printing. BAP was supported by a post-doctoral grant within the FCT funded project PTDC/BBB-BEP/0869/2014. This work was also funded by national funds (OE), through FCT, I.P., within the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. AC was supported by FCT through Stimulus of Scientific Employment - Individual Support 2017 (CEECIND/01514/2017).
PY - 2019/10/15
Y1 - 2019/10/15
N2 - The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.
AB - The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (β1→3, β1→4)-glucans, (α1→3)-, (α1→4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.
KW - Carbohydrate microarrays
KW - Immunity
KW - Mannans
KW - Mixed-linked glucans
KW - Rhamnans
KW - Sulphated polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=85067212402&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2019.06.001
DO - 10.1016/j.carbpol.2019.06.001
M3 - Article
C2 - 31320077
AN - SCOPUS:85067212402
SN - 0144-8617
VL - 222
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 114962
ER -