TY - JOUR
T1 - Stem cells characterization
T2 - OMICS reinforcing analytics
AU - Sebastião, Maria J.
AU - Serra, Margarida
AU - Gomes-Alves, Patrícia
AU - Alves, Paula M.
N1 - Funding Information:
Authors acknowledge funding from project BRAV3 (H2020, 874827), Funda??o para a Ci?ncia e Tecnologia projects: NETDIAMOND - SAICTPAC/0047/2015, MetaCardio - PTDC/BTMSAL/32566/2017 and iNOVA4Health ? UIDB/04462/2020, UIDP/04462/2020.
Funding Information:
Authors acknowledge funding from project BRAV3 (H2020, 874827 ), Fundação para a Ciência e Tecnologia projects : NETDIAMOND - SAICTPAC/0047/2015, MetaCardio - PTDC/BTMSAL/32566/2017 and iNOVA4Health – UIDB/04462/2020 , UIDP/04462/2020 .
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/10
Y1 - 2021/10
N2 - Stem cells hold outstanding potential to model and treat disease and are valuable tools in pharmacology and toxicology. Characterization of stem cells and derivatives still poses many challenges to ensure safe, efficacious, and reliable therapies. Regulatory agencies have defined key mandatory attributes related to identity, purity, sterility, and genomic integrity, however robust analytics to determine cell's potency are still a major challenge, in most cases assessed case-by-case. Importantly, the application of high-throughput ‘omic tools is opening new perspectives on stem cell's research and development. Here, analytical methodologies currently employed to characterize stem cells’ quality attributes are discussed, with special focus on ‘omics as relevant tools for definition of cell's mechanism of action, and for potency assay development and assessment.
AB - Stem cells hold outstanding potential to model and treat disease and are valuable tools in pharmacology and toxicology. Characterization of stem cells and derivatives still poses many challenges to ensure safe, efficacious, and reliable therapies. Regulatory agencies have defined key mandatory attributes related to identity, purity, sterility, and genomic integrity, however robust analytics to determine cell's potency are still a major challenge, in most cases assessed case-by-case. Importantly, the application of high-throughput ‘omic tools is opening new perspectives on stem cell's research and development. Here, analytical methodologies currently employed to characterize stem cells’ quality attributes are discussed, with special focus on ‘omics as relevant tools for definition of cell's mechanism of action, and for potency assay development and assessment.
UR - http://www.scopus.com/inward/record.url?scp=85113277663&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2021.07.021
DO - 10.1016/j.copbio.2021.07.021
M3 - Review article
C2 - 34425321
AN - SCOPUS:85113277663
SN - 0958-1669
VL - 71
SP - 175
EP - 181
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -