Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection

Pedro Quaresma, Inês Osório, Gonçalo Dória, Patrícia A. Carvalho, André Pereira, Judith Langer, João Pedro Araújo, Isabel Pastoriza-Santos, Luis M. Liz-Marzán, Ricardo Franco, Pedro Baptista, Eulália Pereira

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e. g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).

Original languageEnglish
Pages (from-to)3659-3667
Number of pages9
JournalRCS Advances
Volume4
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Ferrosoferric Oxide
Magnetic separation
Magnetite
Gold
Stars
Raman spectroscopy
Nanoparticles
Proteins
Magnetite Nanoparticles
Magnetite nanoparticles
Histidine
Gold coatings
Maltose-Binding Proteins
Maltose
Nickel
Magnets
Magnetic properties
Coloring Agents
Dyes
Optical properties

Keywords

  • IRON-OXIDE NANOPARTICLES
  • ENHANCED RAMAN-SCATTERING
  • HISTIDINE-TAGGED PROTEINS
  • BIOLOGICAL APPLICATIONS
  • BIOMEDICAL APPLICATIONS
  • SHELL NANOPARTICLES
  • SURFACE
  • NANOSTARS
  • MAGHEMITE
  • SIZE

Cite this

Quaresma, P., Osório, I., Dória, G., Carvalho, P. A., Pereira, A., Langer, J., ... Pereira, E. (2014). Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection. RCS Advances, 4(8), 3659-3667. https://doi.org/10.1039/c3ra46762g
Quaresma, Pedro ; Osório, Inês ; Dória, Gonçalo ; Carvalho, Patrícia A. ; Pereira, André ; Langer, Judith ; Araújo, João Pedro ; Pastoriza-Santos, Isabel ; Liz-Marzán, Luis M. ; Franco, Ricardo ; Baptista, Pedro ; Pereira, Eulália. / Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection. In: RCS Advances. 2014 ; Vol. 4, No. 8. pp. 3659-3667.
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abstract = "A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e. g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).",
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Quaresma, P, Osório, I, Dória, G, Carvalho, PA, Pereira, A, Langer, J, Araújo, JP, Pastoriza-Santos, I, Liz-Marzán, LM, Franco, R, Baptista, P & Pereira, E 2014, 'Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection' RCS Advances, vol. 4, no. 8, pp. 3659-3667. https://doi.org/10.1039/c3ra46762g

Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection. / Quaresma, Pedro; Osório, Inês; Dória, Gonçalo; Carvalho, Patrícia A.; Pereira, André; Langer, Judith; Araújo, João Pedro; Pastoriza-Santos, Isabel; Liz-Marzán, Luis M.; Franco, Ricardo; Baptista, Pedro; Pereira, Eulália.

In: RCS Advances, Vol. 4, No. 8, 2014, p. 3659-3667.

Research output: Contribution to journalArticle

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T1 - Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection

AU - Quaresma, Pedro

AU - Osório, Inês

AU - Dória, Gonçalo

AU - Carvalho, Patrícia A.

AU - Pereira, André

AU - Langer, Judith

AU - Araújo, João Pedro

AU - Pastoriza-Santos, Isabel

AU - Liz-Marzán, Luis M.

AU - Franco, Ricardo

AU - Baptista, Pedro

AU - Pereira, Eulália

N1 - WOS:000329040500001

PY - 2014

Y1 - 2014

N2 - A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e. g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).

AB - A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e. g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).

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KW - HISTIDINE-TAGGED PROTEINS

KW - BIOLOGICAL APPLICATIONS

KW - BIOMEDICAL APPLICATIONS

KW - SHELL NANOPARTICLES

KW - SURFACE

KW - NANOSTARS

KW - MAGHEMITE

KW - SIZE

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U2 - 10.1039/c3ra46762g

DO - 10.1039/c3ra46762g

M3 - Article

VL - 4

SP - 3659

EP - 3667

JO - RCS Advances

JF - RCS Advances

SN - 2046-2069

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ER -

Quaresma P, Osório I, Dória G, Carvalho PA, Pereira A, Langer J et al. Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection. RCS Advances. 2014;4(8):3659-3667. https://doi.org/10.1039/c3ra46762g