Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c

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Abstract

Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.

Original languageEnglish
Pages (from-to)4696-4703
Number of pages8
JournalChemelectrochem
Volume6
Issue number17
DOIs
Publication statusPublished - 2 Sep 2019

Keywords

  • cytochrome c
  • direct electron transfer
  • gold nanostars
  • nanoparticles
  • protein electrochemistry

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