TY - JOUR
T1 - Staphylococcus aureus Infecting and Colonizing Experimental Animals, Macaques, in a Research Animal Facility
AU - De La Gandara, Maria Pardos
AU - Diaz, Leslie
AU - Euler, Chad W.
AU - Chung, Marilyn
AU - Gonzalez, Alejandra
AU - Cheleuitte, Christopher
AU - Freiwald, Winrich
AU - Tomasz, Alexander
AU - Fischetti, Vincent A.
AU - De Lencastre, Hermínia
PY - 2019/1/1
Y1 - 2019/1/1
N2 - An outbreak of methicillin-resistant Staphylococcus aureus (MRSA) infections on the skin and soft tissues of experimental macaques in the vivarium of The Rockefeller University, New York, triggered this observational and interventional study. We screened 14 macaques in the colony (samples from head, nares, and rectum) and their housing (40 environmental surfaces) four times in 1 year, for S. aureus colonization or contamination, while implementing enhanced decolonization and decontamination procedures. A total of 114 isolates of S. aureus were recovered and characterized (antibiograms, spa typing, multilocus sequence typing, pulsed-field gel electrophoresis [PFGE], mecA, Panton-Valentine Leukocidin, and arginine catabolic mobile element). Based on these results, six strains of S. aureus were identified: Two MRSA strains (t16708/ST3862/PFGE-A, t16709/ST3862/PFGE-C) and one methicillin-sensitive S. aureus (t8397/ST3884/PFGE-D) were characterized for the first time in this study; strains belonging to spa types t189 and t4167 have been identified in primates in previous studies. None of these strains was common to the neighboring New York City human community. Thus, it seems probable that the animals were already colonized upon arrival to the University. We suggest screening primates for S. aureus carriage upon arrival to University vivaria and possible implementation of extensive decolonization procedures before any surgical interventions.
AB - An outbreak of methicillin-resistant Staphylococcus aureus (MRSA) infections on the skin and soft tissues of experimental macaques in the vivarium of The Rockefeller University, New York, triggered this observational and interventional study. We screened 14 macaques in the colony (samples from head, nares, and rectum) and their housing (40 environmental surfaces) four times in 1 year, for S. aureus colonization or contamination, while implementing enhanced decolonization and decontamination procedures. A total of 114 isolates of S. aureus were recovered and characterized (antibiograms, spa typing, multilocus sequence typing, pulsed-field gel electrophoresis [PFGE], mecA, Panton-Valentine Leukocidin, and arginine catabolic mobile element). Based on these results, six strains of S. aureus were identified: Two MRSA strains (t16708/ST3862/PFGE-A, t16709/ST3862/PFGE-C) and one methicillin-sensitive S. aureus (t8397/ST3884/PFGE-D) were characterized for the first time in this study; strains belonging to spa types t189 and t4167 have been identified in primates in previous studies. None of these strains was common to the neighboring New York City human community. Thus, it seems probable that the animals were already colonized upon arrival to the University. We suggest screening primates for S. aureus carriage upon arrival to University vivaria and possible implementation of extensive decolonization procedures before any surgical interventions.
KW - MRSA
KW - Staphylococcus aureus
KW - veterinary infections
UR - http://www.scopus.com/inward/record.url?scp=85059797920&partnerID=8YFLogxK
U2 - 10.1089/mdr.2018.0232
DO - 10.1089/mdr.2018.0232
M3 - Article
C2 - 30481118
AN - SCOPUS:85059797920
SN - 1076-6294
VL - 25
SP - 54
EP - 62
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 1
ER -
