Standard key steps in mass spectrometry-based plant metabolomics experiments: Instrument performance and analytical method validation

Ana Margarida Rodrigues, Carla António

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physicochemical properties requiring powerful analytical tools for the separation, characterization, and quantification of this vast compound diversity present in plant matrices. In quantitative metabolomics studies, major efforts are put into optimizing sample extraction and separation as well as instrument conditions to measure specific plant metabolites. Here, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and an overview of the most critical steps in the development and validation of MS-based analytical methods is presented.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-31
Number of pages13
Volume1778
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1778
ISSN (Print)1064-3745

Keywords

  • Analytical recoveries
  • GC-MS
  • LC-MS
  • Mass spectrometry
  • Matrix effects
  • Method validation
  • Stable-isotope-labeled standards

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