Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands

Josue Carvalho, Joao Ferreira, P. Pereira, E. Coutinho, Aurore Guedin, Pierre Nottelet, Gilmar E. Salgado, Jean-Louis Mergny, J. A. Queiroz, F. Sousa, Eurico J. Cabrita, C. Cruz

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6 Citations (Scopus)

Abstract

The topological study of novel quadruplex-forming DNA sequences found in immunoglobulin switch regions S mu and S gamma 3 is described along with the synthesis and characterization of naphthalene and quinoline-based ligands for the G-quadruplex binding and stabilization. Several biophysical and biochemical techniques are employed to assess the G-quadruplex formation and stabilization by the ligands. The results indicate that 58S gamma 3 folds into a parallel-stranded G-quadruplex structure in K+ buffer while 33S mu folds into a hybrid-type G-quadruplex in the same buffer. The novel naphthalene and quinoline-based ligands bind and stabilize specifically a variety of G-quadruplexes. This study provides evidence of the importance of G-quadruplex structures to the biology of immunoglobulin class switch recombination. (C) 2016 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1229-1237
Number of pages9
JournalTetrahedron
Volume72
Issue number9
DOIs
Publication statusPublished - 3 Mar 2016

Keywords

  • G-quadruplex ligands
  • Circular dichroism
  • Thermal difference spectra
  • FRET melting
  • PCR-stop
  • G4-FID

Cite this

Carvalho, J., Ferreira, J., Pereira, P., Coutinho, E., Guedin, A., Nottelet, P., ... Cruz, C. (2016). Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands. Tetrahedron, 72(9), 1229-1237. https://doi.org/10.1016/j.tet.2016.01.022