Stability of anthocyanins extracted from grape skins

Helena Morais, Cristina Ramos, E. Forgasc, Tibor Cserhàti, Natalia Matos, Vitor Almeida, José Santos Oliveira

Research output: Contribution to journalConference articlepeer-review

17 Citations (Scopus)


The extractability and stability of anthocyanins from the skins of Vitis vinifera were determined at different pH values. Anthocyanins were extracted using acetone, partitioned with chloroform and pre-purified by solid-phase extraction (SPE). They were analysed by RP-HPLC, and the kinetic parameters of decomposition were calculated. The total monomeric anthocyanin content was determined by spectrophotometry Anthocianins were well separated by RP-HPLC. The efficiency of extraction depended strongly on the pH of the extracting agent and on the character of the pigment to be extracted. The amount of anthocyanins decreased with increasing duration of storage, more so at elevated temperatures.
Original languageEnglish
Pages (from-to)S173-S175
Number of pages3
Issue numberSuppl. S
Publication statusPublished - 1 Jan 2002
Event4th Balaton Symposium on High-Performance Separation Methods - Siofok, Hungary
Duration: 2 Sep 20014 Sep 2001


  • Column liquid chromatography
  • Kinetic parameters
  • Anthocyanins
  • Degradation reactions


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