Stability of anthocyanins extracted from grape skins

Helena Morais; Cristina Ramos; E. Forgasc; Tibor Cserhàti; Natalia Matos; Vitor Almeida; José Santos Oliveira

doi:10.1007/BF02494133

Stability of anthocyanins extracted from grape skins

Helena Morais, Cristina Ramos, E. Forgasc, Tibor Cserhàti, Natalia Matos, Vitor Almeida, José Santos Oliveira

DCTB - Departamento de Ciências e Tecnologia da Biomassa (ex-GDEH)

Research output: Contribution to journal › Conference article

17 Citations (Scopus)

Abstract

The extractability and stability of anthocyanins from the skins of Vitis vinifera were determined at different pH values. Anthocyanins were extracted using acetone, partitioned with chloroform and pre-purified by solid-phase extraction (SPE). They were analysed by RP-HPLC, and the kinetic parameters of decomposition were calculated. The total monomeric anthocyanin content was determined by spectrophotometry Anthocianins were well separated by RP-HPLC. The efficiency of extraction depended strongly on the pH of the extracting agent and on the character of the pigment to be extracted. The amount of anthocyanins decreased with increasing duration of storage, more so at elevated temperatures.

Original language	English
Pages (from-to)	S173-S175
Number of pages	3
Journal	Chromatographia
Volume	56
Issue number	Suppl. S
DOIs	https://doi.org/10.1007/BF02494133
Publication status	Published - 1 Jan 2002
Event	4th Balaton Symposium on High-Performance Separation Methods - Siofok, Hungary Duration: 2 Sep 2001 → 4 Sep 2001

Keywords

Column liquid chromatography
Kinetic parameters
Anthocyanins
Degradation reactions

Access to Document

10.1007/BF02494133

https://www.scopus.com/record/display.uri?eid=2-s2.0-0036062759&origin=resultslist&sort=plf-f&src=s&st1

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title = "Stability of anthocyanins extracted from grape skins",

abstract = "The extractability and stability of anthocyanins from the skins of Vitis vinifera were determined at different pH values. Anthocyanins were extracted using acetone, partitioned with chloroform and pre-purified by solid-phase extraction (SPE). They were analysed by RP-HPLC, and the kinetic parameters of decomposition were calculated. The total monomeric anthocyanin content was determined by spectrophotometry Anthocianins were well separated by RP-HPLC. The efficiency of extraction depended strongly on the pH of the extracting agent and on the character of the pigment to be extracted. The amount of anthocyanins decreased with increasing duration of storage, more so at elevated temperatures.",

keywords = "anthocyanins, degradation reactions, column liquid chromatography, kinetic parameters, Column liquid chromatography, Kinetic parameters, Anthocyanins, Degradation reactions",

author = "Helena Morais and Cristina Ramos and E. Forgasc and Tibor Cserh{\`a}ti and Natalia Matos and Vitor Almeida and Oliveira, {Jos{\'e} Santos}",

year = "2002",

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doi = "10.1007/BF02494133",

language = "English",

volume = "56",

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note = "4th Balaton Symposium on High-Performance Separation Methods ; Conference date: 02-09-2001 Through 04-09-2001",

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T1 - Stability of anthocyanins extracted from grape skins

AU - Morais, Helena

AU - Ramos, Cristina

AU - Forgasc, E.

AU - Cserhàti, Tibor

AU - Matos, Natalia

AU - Almeida, Vitor

AU - Oliveira, José Santos

PY - 2002/1/1

Y1 - 2002/1/1

N2 - The extractability and stability of anthocyanins from the skins of Vitis vinifera were determined at different pH values. Anthocyanins were extracted using acetone, partitioned with chloroform and pre-purified by solid-phase extraction (SPE). They were analysed by RP-HPLC, and the kinetic parameters of decomposition were calculated. The total monomeric anthocyanin content was determined by spectrophotometry Anthocianins were well separated by RP-HPLC. The efficiency of extraction depended strongly on the pH of the extracting agent and on the character of the pigment to be extracted. The amount of anthocyanins decreased with increasing duration of storage, more so at elevated temperatures.

AB - The extractability and stability of anthocyanins from the skins of Vitis vinifera were determined at different pH values. Anthocyanins were extracted using acetone, partitioned with chloroform and pre-purified by solid-phase extraction (SPE). They were analysed by RP-HPLC, and the kinetic parameters of decomposition were calculated. The total monomeric anthocyanin content was determined by spectrophotometry Anthocianins were well separated by RP-HPLC. The efficiency of extraction depended strongly on the pH of the extracting agent and on the character of the pigment to be extracted. The amount of anthocyanins decreased with increasing duration of storage, more so at elevated temperatures.

KW - anthocyanins

KW - degradation reactions

KW - column liquid chromatography

KW - kinetic parameters

KW - Column liquid chromatography

KW - Kinetic parameters

KW - Anthocyanins

KW - Degradation reactions

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DO - 10.1007/BF02494133

M3 - Conference article

VL - 56

SP - S173-S175

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T2 - 4th Balaton Symposium on High-Performance Separation Methods

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