SraL sRNA interaction regulates the terminator by preventing premature transcription termination of rho mRNA

Inês Jesus Silva, Susana Barahona, Alex Eyraud, David Lalaouna, Nara Figueroa-Bossi, Eric Massé, Cecília Maria Arraiano

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Transcription termination is a critical step in the control of gene expression. One of the major termination mechanisms is mediated by Rho factor that dissociates the complex mRNA-DNA-RNA polymerase upon binding with RNA polymerase. Rho promotes termination at the end of operons, but it can also terminate transcription within leader regions, performing regulatory functions and avoiding pervasive transcription. Transcription of rho is autoregulated through a Rho-dependent attenuation in the leader region of the transcript. In this study, we have included an additional player in this pathway. By performing MS2-affinity purification coupled with RNA sequencing (MAPS), rho transcript was shown to directly interact with the small noncoding RNA SraL. Using bioinformatic in vivo and in vitro experimental analyses, SraL was shown to base pair with the 5′-UTR of rho mRNA upregulating its expression in several growth conditions. This base pairing was shown to prevent the action of Rho over its own message. Moreover, the results obtained indicate that both ProQ and Hfq are associated with this regulation. We propose a model that contemplates the action of Salmonella SraL sRNA in the protection of rho mRNA from premature transcription termination by Rho. Note that since the interaction region between both RNAs corresponds to a very-well-conserved sequence, it is plausible to admit that this regulation also occurs in other enterobacteria.

Original languageEnglish
Pages (from-to)3042-3051
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number8
DOIs
Publication statusPublished - 19 Feb 2019

Keywords

  • Functional regulatory RNA
  • Posttranscriptional control
  • Prokaryotes
  • Small noncoding RNA
  • Transcription termination

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