TY - JOUR
T1 - Sporobolomyces agrorum sp. Nov. and sporobolomyces sucorum sp. nov., two novel basidiomycetous yeast species isolated from grape and apple must in italy
AU - Lorenzini, Marilinda
AU - Zapparoli, Giacomo
AU - Azzolini, Michela
AU - Carvalho, Claudia
AU - Sampaio, Jose Paulo
N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT#
PY - 2019/11/1
Y1 - 2019/11/1
N2 - During a survey of yeast populations associated with grape and apple musts used for wine and cider fermentation, respectively, six pink-coloured ballistoconidia-forming yeasts belonging to the order Sporidiobolales (Basidiomycota) were isolated. Phylogenetic analysis inferred using sequences of the internal transcribed spacer (ITS), the D1/D2 domain of the large subunit rRNA gene, the small subunit (SSU) rRNA gene and DNA-directed RNA polymerase II subunit (RPB2) indicated that the six isolates were separated in two novel species. One of the new species, Sporobolomyces agrorum sp. nov., isolated from grape must, had Sporobolomyces roseus and Sporobolomyces metaroseus as its closest relatives, but showed four/two and 16 nucleotide substitutions in the D1/D2 and ITS regions, respectively, to these two species. The other novel species, Sporobolomyces sucorum sp. nov., was found in apple must and was closely related to Sporobolomyces pararoseus and Sporobolomyces patagonicus, but showed two/three and five substitutions in those two regions for its closest relatives. We detected additional representatives of this species, most of them isolated from grapes whose sequences were already available on public databases. A sexual stage could not be observed for the novel species.
AB - During a survey of yeast populations associated with grape and apple musts used for wine and cider fermentation, respectively, six pink-coloured ballistoconidia-forming yeasts belonging to the order Sporidiobolales (Basidiomycota) were isolated. Phylogenetic analysis inferred using sequences of the internal transcribed spacer (ITS), the D1/D2 domain of the large subunit rRNA gene, the small subunit (SSU) rRNA gene and DNA-directed RNA polymerase II subunit (RPB2) indicated that the six isolates were separated in two novel species. One of the new species, Sporobolomyces agrorum sp. nov., isolated from grape must, had Sporobolomyces roseus and Sporobolomyces metaroseus as its closest relatives, but showed four/two and 16 nucleotide substitutions in the D1/D2 and ITS regions, respectively, to these two species. The other novel species, Sporobolomyces sucorum sp. nov., was found in apple must and was closely related to Sporobolomyces pararoseus and Sporobolomyces patagonicus, but showed two/three and five substitutions in those two regions for its closest relatives. We detected additional representatives of this species, most of them isolated from grapes whose sequences were already available on public databases. A sexual stage could not be observed for the novel species.
KW - Apple juice
KW - Basidiomycetous yeast
KW - Grape must
KW - Sporobolomyces agrorum
KW - Sporobolomyces sucorum
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85074446601&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003626
DO - 10.1099/ijsem.0.003626
M3 - Article
C2 - 31368884
AN - SCOPUS:85074446601
SN - 1466-5026
VL - 69
SP - 3385
EP - 3391
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
M1 - 3626
ER -