SPIM-fluid: Open source light-sheet based platform for high-throughput imaging

Emilio J. Gualda; Hugo Pereira; Tiago Vale; Marta Falcão Estrada; Ana Catarina Montes; Nuno Moreno

doi:10.1364/BOE.6.004447

SPIM-fluid: Open source light-sheet based platform for high-throughput imaging

Emilio J. Gualda, Hugo Pereira, Tiago Vale, Marta Falcão Estrada, Ana Catarina Montes, Nuno Moreno

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article

33 Citations (Scopus)

Abstract

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.

Original language	English
Article number	245811
Pages (from-to)	4447-4456
Number of pages	10
Journal	Biomedical Optics Express
Volume	6
Issue number	11
DOIs	https://doi.org/10.1364/BOE.6.004447
Publication status	Published - 19 Oct 2015

Keywords

Fluorescence microscopy
Three-dimensional image acquisition

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10.1364/BOE.6.004447

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AU - Moreno, Nuno

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