Abstract
Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.
Original language | English |
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Article number | 245811 |
Pages (from-to) | 4447-4456 |
Number of pages | 10 |
Journal | Biomedical Optics Express |
Volume | 6 |
Issue number | 11 |
DOIs | |
Publication status | Published - 19 Oct 2015 |
Keywords
- Fluorescence microscopy
- Three-dimensional image acquisition