SPIM-fluid: Open source light-sheet based platform for high-throughput imaging

Emilio J. Gualda, Hugo Pereira, Tiago Vale, Marta Falcão Estrada, Ana Catarina Montes, Nuno Moreno

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.

Original languageEnglish
Article number245811
Pages (from-to)4447-4456
Number of pages10
JournalBiomedical Optics Express
Issue number11
Publication statusPublished - 19 Oct 2015


  • Fluorescence microscopy
  • Three-dimensional image acquisition


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