Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylyl-sulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.

Original languageEnglish
Pages (from-to)909-912
Number of pages4
JournalJournal Of Proteome Research
Volume6
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007

Keywords

  • MALDI-TOF-MS
  • Protein digestion
  • Sonoreactor

Fingerprint Dive into the research topics of 'Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins'. Together they form a unique fingerprint.

  • Cite this