Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens

Hassiba Metrouh-Amir, Catarina M M Duarte, Fadila Maiza

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Matricaria Pubescens (Asteraceae) is a well known medicinal plant used in the south west of Algeria against several diseases. This study was devoted to the determination of polyphenols, flavonoids, and condensed tannins contents of M. pubescens after their extraction using various solvents with different polarities (distilled water, aqueous acetone (50%), aqueous methanol (50%), aqueous ethanol (50%), acetone (100%), methanol (100%), and ethanol (100%)), as well as the determination of the antioxidant and the antibacterial activities of all obtained extracts. The results obtained indicate that the aqueous methanol (50%) and the aqueous ethanol (50%) extracts presented the highest contents of polyphenols. The antioxidant activity was evaluated using ferric reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, oxygen radical absorbance capacity (ORAC) and hydroxyl radical scavenging capacity (HOSC). The results showed that all extracts of M. pubescens, prepared using different solvent, have diverse antioxidant capacities, however aqueous methanol (50%), aqueous ethanol (50%) and aqueous acetone (50%) exhibited the highest antioxidant capacity. Extracts of M. pubescens exhibited an inhibiting capacity against the tested strains except: Escherichia coli ATCC 25922 and Acinetobacter baumannii 897. The aqueous methanol (50%) and the aqueous ethanol (50%) extracts were most effective; the greatest zone of inhibition was obtained with the aqueous ethanol (50%) extract against Methicillin-resistant Staphylococcus aureuscStaphylococcus aureusc. The minimum inhibitory concentration (MIC) with the value of 0.78. mg/mL obtained against A. baumannii 610 using the aqueous methanol (50%) extract indicate that this plant is a potential natural antimicrobial agent. These biological activities could support the traditional use of M. pubescens.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalIndustrial Crops and Products
Volume67
DOIs
Publication statusPublished - 1 May 2015

Fingerprint

Matricaria
antioxidants
water
methanol
ethanol
extracts
Acinetobacter baumannii
acetone
polyphenols
oxygen radical absorbance capacity
methicillin
Algeria
hydroxyl radicals
minimum inhibitory concentration
proanthocyanidins
Staphylococcus
plant extracts

Keywords

  • Antibacterial activity
  • Antioxidant Activity
  • Condensed tannins
  • Flavonoids
  • Matricaria pubescens
  • Polyphenols

Cite this

@article{588e65cbd18b402cab41905328ad13dd,
title = "Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens",
abstract = "Matricaria Pubescens (Asteraceae) is a well known medicinal plant used in the south west of Algeria against several diseases. This study was devoted to the determination of polyphenols, flavonoids, and condensed tannins contents of M. pubescens after their extraction using various solvents with different polarities (distilled water, aqueous acetone (50{\%}), aqueous methanol (50{\%}), aqueous ethanol (50{\%}), acetone (100{\%}), methanol (100{\%}), and ethanol (100{\%})), as well as the determination of the antioxidant and the antibacterial activities of all obtained extracts. The results obtained indicate that the aqueous methanol (50{\%}) and the aqueous ethanol (50{\%}) extracts presented the highest contents of polyphenols. The antioxidant activity was evaluated using ferric reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, oxygen radical absorbance capacity (ORAC) and hydroxyl radical scavenging capacity (HOSC). The results showed that all extracts of M. pubescens, prepared using different solvent, have diverse antioxidant capacities, however aqueous methanol (50{\%}), aqueous ethanol (50{\%}) and aqueous acetone (50{\%}) exhibited the highest antioxidant capacity. Extracts of M. pubescens exhibited an inhibiting capacity against the tested strains except: Escherichia coli ATCC 25922 and Acinetobacter baumannii 897. The aqueous methanol (50{\%}) and the aqueous ethanol (50{\%}) extracts were most effective; the greatest zone of inhibition was obtained with the aqueous ethanol (50{\%}) extract against Methicillin-resistant Staphylococcus aureuscStaphylococcus aureusc. The minimum inhibitory concentration (MIC) with the value of 0.78. mg/mL obtained against A. baumannii 610 using the aqueous methanol (50{\%}) extract indicate that this plant is a potential natural antimicrobial agent. These biological activities could support the traditional use of M. pubescens.",
keywords = "Antibacterial activity, Antioxidant Activity, Condensed tannins, Flavonoids, Matricaria pubescens, Polyphenols",
author = "Hassiba Metrouh-Amir and Duarte, {Catarina M M} and Fadila Maiza",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.indcrop.2015.01.049",
language = "English",
volume = "67",
pages = "249--256",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier Science B.V., Inc",

}

Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens. / Metrouh-Amir, Hassiba; Duarte, Catarina M M; Maiza, Fadila.

In: Industrial Crops and Products, Vol. 67, 01.05.2015, p. 249-256.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens

AU - Metrouh-Amir, Hassiba

AU - Duarte, Catarina M M

AU - Maiza, Fadila

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Matricaria Pubescens (Asteraceae) is a well known medicinal plant used in the south west of Algeria against several diseases. This study was devoted to the determination of polyphenols, flavonoids, and condensed tannins contents of M. pubescens after their extraction using various solvents with different polarities (distilled water, aqueous acetone (50%), aqueous methanol (50%), aqueous ethanol (50%), acetone (100%), methanol (100%), and ethanol (100%)), as well as the determination of the antioxidant and the antibacterial activities of all obtained extracts. The results obtained indicate that the aqueous methanol (50%) and the aqueous ethanol (50%) extracts presented the highest contents of polyphenols. The antioxidant activity was evaluated using ferric reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, oxygen radical absorbance capacity (ORAC) and hydroxyl radical scavenging capacity (HOSC). The results showed that all extracts of M. pubescens, prepared using different solvent, have diverse antioxidant capacities, however aqueous methanol (50%), aqueous ethanol (50%) and aqueous acetone (50%) exhibited the highest antioxidant capacity. Extracts of M. pubescens exhibited an inhibiting capacity against the tested strains except: Escherichia coli ATCC 25922 and Acinetobacter baumannii 897. The aqueous methanol (50%) and the aqueous ethanol (50%) extracts were most effective; the greatest zone of inhibition was obtained with the aqueous ethanol (50%) extract against Methicillin-resistant Staphylococcus aureuscStaphylococcus aureusc. The minimum inhibitory concentration (MIC) with the value of 0.78. mg/mL obtained against A. baumannii 610 using the aqueous methanol (50%) extract indicate that this plant is a potential natural antimicrobial agent. These biological activities could support the traditional use of M. pubescens.

AB - Matricaria Pubescens (Asteraceae) is a well known medicinal plant used in the south west of Algeria against several diseases. This study was devoted to the determination of polyphenols, flavonoids, and condensed tannins contents of M. pubescens after their extraction using various solvents with different polarities (distilled water, aqueous acetone (50%), aqueous methanol (50%), aqueous ethanol (50%), acetone (100%), methanol (100%), and ethanol (100%)), as well as the determination of the antioxidant and the antibacterial activities of all obtained extracts. The results obtained indicate that the aqueous methanol (50%) and the aqueous ethanol (50%) extracts presented the highest contents of polyphenols. The antioxidant activity was evaluated using ferric reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity, oxygen radical absorbance capacity (ORAC) and hydroxyl radical scavenging capacity (HOSC). The results showed that all extracts of M. pubescens, prepared using different solvent, have diverse antioxidant capacities, however aqueous methanol (50%), aqueous ethanol (50%) and aqueous acetone (50%) exhibited the highest antioxidant capacity. Extracts of M. pubescens exhibited an inhibiting capacity against the tested strains except: Escherichia coli ATCC 25922 and Acinetobacter baumannii 897. The aqueous methanol (50%) and the aqueous ethanol (50%) extracts were most effective; the greatest zone of inhibition was obtained with the aqueous ethanol (50%) extract against Methicillin-resistant Staphylococcus aureuscStaphylococcus aureusc. The minimum inhibitory concentration (MIC) with the value of 0.78. mg/mL obtained against A. baumannii 610 using the aqueous methanol (50%) extract indicate that this plant is a potential natural antimicrobial agent. These biological activities could support the traditional use of M. pubescens.

KW - Antibacterial activity

KW - Antioxidant Activity

KW - Condensed tannins

KW - Flavonoids

KW - Matricaria pubescens

KW - Polyphenols

UR - http://www.scopus.com/inward/record.url?scp=84922246460&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2015.01.049

DO - 10.1016/j.indcrop.2015.01.049

M3 - Article

VL - 67

SP - 249

EP - 256

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -