Solution structures of tetrahaem ferricytochrome c(3) from Desulfovibrio vulgaris (Hildenborough) and its K45Q mutant: The molecular basis of cooperativity

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Abstract

The NMR structure of the oxidised wild-type cytochrome c(3) from Desulfovibrio vulgaris Hildenborough was determined in solution. Using a newly developed methodology, NMR data from the K45Q mutant was then grafted onto data from the wild-type protein to determine the structure in the region of the mutation. The structural origins of the redox-Bohr effect and haem-haem cooperativities are discussed with respect to the redox-related conformational changes observed in solution. (C) 2006 Elsevier B.V. All rights reserved.
Original languageUnknown
Pages (from-to)143-153
JournalBiochimica Et Biophysica Acta-Bioenergetics
Volume1757
Issue number2
DOIs
Publication statusPublished - 1 Jan 2006

Keywords

    Cite this

    @article{fb3692b4a618448abe230487b15bb68f,
    title = "Solution structures of tetrahaem ferricytochrome c(3) from Desulfovibrio vulgaris (Hildenborough) and its K45Q mutant: The molecular basis of cooperativity",
    abstract = "The NMR structure of the oxidised wild-type cytochrome c(3) from Desulfovibrio vulgaris Hildenborough was determined in solution. Using a newly developed methodology, NMR data from the K45Q mutant was then grafted onto data from the wild-type protein to determine the structure in the region of the mutation. The structural origins of the redox-Bohr effect and haem-haem cooperativities are discussed with respect to the redox-related conformational changes observed in solution. (C) 2006 Elsevier B.V. All rights reserved.",
    keywords = "AXIAL LIGAND ORIENTATION, PROTEIN STRUCTURES, NUCLEAR-MAGNETIC-RESONANCE, CROSS-RELAXATION, ANGSTROM RESOLUTION, TOCSY EXPERIMENTS, H-1-NMR SPECTRA, BIOLOGICAL MACROMOLECULES, ELECTRONIC-STRUCTURE, CYTOCHROME C(3)",
    author = "Salgueiro, {Carlos Alberto Gomes} and Teixeira, {Ligia Raquel} and Turner, {David Leslie}",
    year = "2006",
    month = "1",
    day = "1",
    doi = "10.1016/j.bbabio.2006.01.007",
    language = "Unknown",
    volume = "1757",
    pages = "143--153",
    journal = "Biochimica Et Biophysica Acta-Bioenergetics",
    issn = "0005-2728",
    publisher = "Elsevier Science B.V., Inc",
    number = "2",

    }

    TY - JOUR

    T1 - Solution structures of tetrahaem ferricytochrome c(3) from Desulfovibrio vulgaris (Hildenborough) and its K45Q mutant: The molecular basis of cooperativity

    AU - Salgueiro, Carlos Alberto Gomes

    AU - Teixeira, Ligia Raquel

    AU - Turner, David Leslie

    PY - 2006/1/1

    Y1 - 2006/1/1

    N2 - The NMR structure of the oxidised wild-type cytochrome c(3) from Desulfovibrio vulgaris Hildenborough was determined in solution. Using a newly developed methodology, NMR data from the K45Q mutant was then grafted onto data from the wild-type protein to determine the structure in the region of the mutation. The structural origins of the redox-Bohr effect and haem-haem cooperativities are discussed with respect to the redox-related conformational changes observed in solution. (C) 2006 Elsevier B.V. All rights reserved.

    AB - The NMR structure of the oxidised wild-type cytochrome c(3) from Desulfovibrio vulgaris Hildenborough was determined in solution. Using a newly developed methodology, NMR data from the K45Q mutant was then grafted onto data from the wild-type protein to determine the structure in the region of the mutation. The structural origins of the redox-Bohr effect and haem-haem cooperativities are discussed with respect to the redox-related conformational changes observed in solution. (C) 2006 Elsevier B.V. All rights reserved.

    KW - AXIAL LIGAND ORIENTATION

    KW - PROTEIN STRUCTURES

    KW - NUCLEAR-MAGNETIC-RESONANCE

    KW - CROSS-RELAXATION

    KW - ANGSTROM RESOLUTION

    KW - TOCSY EXPERIMENTS

    KW - H-1-NMR SPECTRA

    KW - BIOLOGICAL MACROMOLECULES

    KW - ELECTRONIC-STRUCTURE

    KW - CYTOCHROME C(3)

    U2 - 10.1016/j.bbabio.2006.01.007

    DO - 10.1016/j.bbabio.2006.01.007

    M3 - Article

    VL - 1757

    SP - 143

    EP - 153

    JO - Biochimica Et Biophysica Acta-Bioenergetics

    JF - Biochimica Et Biophysica Acta-Bioenergetics

    SN - 0005-2728

    IS - 2

    ER -