Solution properties of an exopolysaccharide from a Pseudomonas strain obtained using glycerol as sole carbon source

Loic Hilliou, Filomena Freitas, Rui Manuel Freitas Oliveira, Maria D'ascensão Carvalho Fernandes Miranda Reis, David Lespineux, Christian Grandfils, Vitor D. Alves

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We report the solution properties of a new exopolysaccharide (EPS) obtained from a Pseudomonas strain fed with glycerol as the sole source of carbon. This high molecular mass (3 x 10(6) g mol(-1)) biopolymer is essentially made of galactose monomers with pyruvate and succinate groups imparting a polyelectrolyte character. The Smidsrod parameter B computed from the ionic strength dependence of the intrinsic viscosity indicates that the EPS backbone is rather flexible. In salt free aqueous solutions, the zero shear viscosity scaling with concentration follows a typical polyelectrolyte behavior in bad solvent, whereas at high ionic strength the theological response is reminiscent from neutral polymers. Light scattering data indicate that the EPS adopts a globular conformation as a result of hydrophobic interactions. EPS solutions are stable within 4 days as particle sizing does not indicate EPS aggregation. Both globular conformation and stability against precipitation from solution are attributed to the low charge density of the polyelectrolyte. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)526-532
JournalCarbohydrate Polymers
Volume78
Issue number3
DOIs
Publication statusPublished - 15 Oct 2009

Keywords

  • Exopolysaccharide (EPS)
  • Glycerol
  • Light scattering
  • Polyelectrolyte
  • Viscosity

Fingerprint Dive into the research topics of 'Solution properties of an exopolysaccharide from a Pseudomonas strain obtained using glycerol as sole carbon source'. Together they form a unique fingerprint.

  • Cite this