Solubility of amphiphiles in membranes: Influence of phase properties and amphiphile head group

Luís M.B.B. Estronca, Maria João Moreno, Magda S.C. Abreu, Eurico Melo, Winchil L.C. Vaz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The solubilities of two fluorescent lipid amphiphiles with comparable apolar structures and different polar head groups, NBD-hexadecylamine and RG-tetradecylamine (or -octadecylamine), were compared in lipid bilayers at a molar ratio of 1/50 at 23°C. Bilayers examined were in the solid, liquid-disordered, or liquid-ordered phases. While NBD-hexadecylamine was soluble in all the examined bilayer membrane phases, RG-tetradecylamine was stably soluble only in the liquid-disordered phase. RG-tetradecylamine insolubility in solid and liquid-ordered phases manifests itself as an aggregation of the amphiphile over a period of several days and the kinetics of aggregation were studied. Solubility of these amphiphiles in the different phases examined seems to be related to the dipole moment of the amphiphile (in particular, of the polar fluorophore) and its orientation relative to the dipolar potential of the membrane. We propose that amphiphilic molecules inserted into membranes (including lipid-attached proteins) partition into different coexisting membrane phases based upon: (1) nature of the apolar structure (chain length, degree of saturation, and chain branching as has been proposed in the literature); (2) magnitude and orientation of the dipole moment of the polar portion of the molecules relative to the membrane dipolar potential; and (3) hydration forces that are a consequence of ordering of water dipoles at the membrane surface.

Original languageEnglish
Pages (from-to)596-603
Number of pages8
JournalBiochemical And Biophysical Research Communications
Volume296
Issue number3
DOIs
Publication statusPublished - 14 Oct 2002

Keywords

  • Amphiphile aggregation
  • Dipolar surface potential
  • Fluorescent lipid amphiphiles
  • Membrane phases
  • Phase partitioning

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