Simultaneous determination of clopidogrel and its carboxylic acid metabolite by capillary electrophoresis

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Abstract

A capillary electrophoresis method was developed and validated for the first time for the analysis of clopidogrel and its carboxylic acid metabolite Prior to method optimization, the pH dependence of effective mobility of both compounds was determined in order to define the initial pH of the running buffer The optimized method demonstrated to be selective, and linear in the concentration range of 2-100 mu M for both compounds The method limits of detection and quantification were, respectively, 1 2 and 3 7 mu M for clopidogrel and 1 1 and 3 2 mu M for the carboxylic acid metabolite Moreover, method validation demonstrated acceptable results for method repeatability (RSD < 7%), intermediate precision (RSD < 7%) and accuracy (85-96%) and is suitable for the quantitative analysis of clopidogrel and its metabolite in serum samples The validated method was also applied to the determination of the kinetic parameters of the enzymatic hydrolysis of clopidogrel An apparent K-m of 145 +/- 30 mu M and V-max of 0 4, 1.5 and 3.4 mu M/min, respectively for the enzyme concentrations 1 0,2 0 and 4 0 U/ml. were obtained
Original languageUnknown
Pages (from-to)1480-1486
JournalJournal Of Chromatography B-Analytical Technologies In The Biomedical And L
Volume878
Issue number19
DOIs
Publication statusPublished - 1 Jan 2010

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