TY - JOUR
T1 - Settled dust assessment in clinical environment
T2 - useful for the evaluation of a wider bioburden spectrum
AU - Viegas, Carla
AU - Almeida, Beatriz
AU - Monteiro, Ana
AU - Paciência, Inês
AU - Rufo, João Cavaleiro
AU - Carolino, Elisabete
AU - Quintal-Gomes, Anita
AU - Twarużek, Magdalena
AU - Kosicki, Robert
AU - Marchand, Geneviéve
AU - Aranha Caetano, Liliana
AU - Viegas, Susana
PY - 2021
Y1 - 2021
N2 - The collection and analysis of settled dust samples from indoor environments has become one of several environmental sampling methods used to assess bioburden indoors. The aim of the study was to characterize the bioburden in vacuumed settled dust from 10 Primary Health Care Centers by culture based and molecular methods. Results for bacterial load ranged from 1 to 12 CFU.g−1 of dust and Gram-negative bacteria ranged between 1 to 344 CFU.g−1 of dust. Fungal load ranged from 0 CFU.g−1 of dust to uncountable. Aspergillus section Fumigati was detected in 4 sampling sites where culture base-methods could not identify this section. Mucorales (Rhizopus sp.) was observed on 1 mg/L voriconazole. Three out of 10 settled dust samples were contaminated by mycotoxins. Settled dust sampling coupled with air sampling in a routine way might provide useful information about bioburden exposure.
AB - The collection and analysis of settled dust samples from indoor environments has become one of several environmental sampling methods used to assess bioburden indoors. The aim of the study was to characterize the bioburden in vacuumed settled dust from 10 Primary Health Care Centers by culture based and molecular methods. Results for bacterial load ranged from 1 to 12 CFU.g−1 of dust and Gram-negative bacteria ranged between 1 to 344 CFU.g−1 of dust. Fungal load ranged from 0 CFU.g−1 of dust to uncountable. Aspergillus section Fumigati was detected in 4 sampling sites where culture base-methods could not identify this section. Mucorales (Rhizopus sp.) was observed on 1 mg/L voriconazole. Three out of 10 settled dust samples were contaminated by mycotoxins. Settled dust sampling coupled with air sampling in a routine way might provide useful information about bioburden exposure.
KW - bioburden
KW - clinical environment
KW - exposure assessment
KW - mycotoxins
KW - Settled dust
UR - http://www.scopus.com/inward/record.url?scp=85068224098&partnerID=8YFLogxK
U2 - 10.1080/09603123.2019.1634799
DO - 10.1080/09603123.2019.1634799
M3 - Article
C2 - 31240954
AN - SCOPUS:85068224098
SN - 0960-3123
VL - 31
SP - 160
EP - 178
JO - International Journal of Environmental Health Research
JF - International Journal of Environmental Health Research
IS - 2
ER -
