Serum adipokine profile and fatty acid composition of adipose tissues are affected by conjugated linoleic acid and saturated fat diets in obese Zucker rats

S.V. Martins, P.A. Lopes, C.M. Alfaia, P.O. Rodrigues, S.P. Alves, R.M.A. Pinto, M.F. Castro, R.J.B. Bessa, J.A.M. Prates

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Abstract

Conjugated linoleic acid (CLA) has been reported as having body fat lowering properties and the ability to modulate the inflammatory system in several models. In the present study, the effects of CLA added to saturated fat diets, from vegetable and animal origins, on the serum adipokine profile of obese Zucker rats were assessed. In addition, the fatty acid composition of epididymal and retroperitoneal adipose tissues was determined and a principal component analysis (PCA) was used to assess possible relationships between fatty acids and serum metabolites. Atherogenic diets (2% cholesterol) were formulated with palm oil and ovine fat and supplemented or not with 1% of a mixture (1:1) of cis-9, trans-11 and trans-10, cis-12-CLA isomers. CLA-fed animals exhibited lower daily feed intake, final body and liver weights, and hepatic lipids content. Total and LDL-cholesterol levels were increased in CLA-supplemented groups. CLA also promoted higher adiponectin and lower plasminogen activator inhibitor-1 (PAI-1) serum concentrations. In contrast to palm oil diets, ovine fat increased insulin resistance and serum levels of leptin, TNF-α and IL-1β. Epididymal and retroperitoneal adipose tissues had similar deposition of individual fatty acids. The PCA analysis showed that the trans-10, cis-12-CLA isomer was highly associated with adiponectin and PAI-1 levels. Summing up, CLA added to vegetable saturated enriched diets, relative to those from animal origin, seems to improve the serum profile of adipokines and inflammatory markers in obese Zucker rats due to a more favourable fatty acid composition. © 2009 The Authors.
Original languageEnglish
Pages (from-to)869-878
Number of pages10
JournalBritish Journal Of Nutrition
Volume103
Issue number6
Early online date2 Nov 2009
DOIs
Publication statusPublished - 28 Mar 2010

Keywords

  • Adipokines
  • Conjugated linoleic acid
  • Fatty acids
  • Obese Zucker rats
  • Saturated diets
  • adipocytokine
  • adiponectin
  • C reactive protein
  • conjugated linoleic acid
  • interleukin 1beta
  • interleukin 6
  • leptin
  • low density lipoprotein cholesterol
  • monounsaturated fatty acid
  • palm oil
  • plasminogen activator inhibitor 1
  • polyunsaturated fatty acid
  • saturated fatty acid
  • tumor necrosis factor alpha
  • cholesterol
  • edible oil
  • fatty acid
  • lipid
  • trans 10,cis 12 conjugated linoleic acid
  • trans-10,cis-12-conjugated linoleic acid
  • vegetable oil
  • animal experiment
  • animal model
  • animal tissue
  • article
  • atherogenic diet
  • body weight
  • cholesterol blood level
  • controlled study
  • epididymis fat
  • inflammation
  • insulin resistance
  • lipid composition
  • lipid liver level
  • lipid storage
  • liver weight
  • male
  • nonhuman
  • obesity
  • principal component analysis
  • rat
  • retroperitoneal fat
  • adipose tissue
  • animal
  • blood
  • body composition
  • chemistry
  • epididymis
  • fat intake
  • histology
  • intraabdominal fat
  • liver
  • metabolism
  • organ size
  • sheep
  • Zucker rat
  • Animalia
  • Ovis
  • Rattus
  • Adipose Tissue
  • Animals
  • Body Composition
  • Cholesterol
  • Dietary Fats
  • Dietary Fats, Unsaturated
  • Epididymis
  • Fatty Acids
  • Intra-Abdominal Fat
  • Linoleic Acids, Conjugated
  • Lipids
  • Liver
  • Male
  • Obesity
  • Organ Size
  • Plant Oils
  • Plasminogen Activator Inhibitor 1
  • Rats
  • Rats, Zucker
  • Sheep

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