TY - JOUR
T1 - Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
T2 - Synthesis, Characterization, and In Vitro Antioxidant Activity
AU - Concórdio-Reis, Patrícia
AU - Macedo, Ana Catarina
AU - Cardeira, Martim
AU - Moppert, Xavier
AU - Guézennec, Jean
AU - Sevrin, Chantal
AU - Grandfils, Christian
AU - Serra, Ana Teresa
AU - Freitas, Filomena
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04462%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04462%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0087%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F131947%2F2017/PT#
Funding Information:
This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledges FCT/MCTES for the Individual Grant CEEC-IND/04801/2017.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/2/2
Y1 - 2023/2/2
N2 - In this study, the novel exopolysaccharide (EPS) produced by the marine bacterium Alteromonas macleodii Mo 169 was used as a stabilizer and capping agent in the preparation of selenium nanoparticles (SeNPs). The synthesized nanoparticles were well dispersed and spherical with an average particle size of 32 nm. The cytotoxicity of the EPS and the EPS/SeNPs bio-nanocomposite was investigated on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines. No cytotoxicity was found for the EPS alone for concentrations up to 1 g L−1. A cytotoxic effect was only noticed for the bio-nanocomposite at the highest concentrations tested (0.5 and 1 g L−1). In vitro experiments demonstrated that non-cytotoxic concentrations of the EPS/SeNPs bio-nanocomposite had a significant cellular antioxidant effect on the HaCaT cell line by reducing ROS levels up to 33.8%. These findings demonstrated that the A. macleodii Mo 169 EPS can be efficiently used as a stabilizer and surface coating to produce a SeNP-based bio-nanocomposite with improved antioxidant activity.
AB - In this study, the novel exopolysaccharide (EPS) produced by the marine bacterium Alteromonas macleodii Mo 169 was used as a stabilizer and capping agent in the preparation of selenium nanoparticles (SeNPs). The synthesized nanoparticles were well dispersed and spherical with an average particle size of 32 nm. The cytotoxicity of the EPS and the EPS/SeNPs bio-nanocomposite was investigated on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines. No cytotoxicity was found for the EPS alone for concentrations up to 1 g L−1. A cytotoxic effect was only noticed for the bio-nanocomposite at the highest concentrations tested (0.5 and 1 g L−1). In vitro experiments demonstrated that non-cytotoxic concentrations of the EPS/SeNPs bio-nanocomposite had a significant cellular antioxidant effect on the HaCaT cell line by reducing ROS levels up to 33.8%. These findings demonstrated that the A. macleodii Mo 169 EPS can be efficiently used as a stabilizer and surface coating to produce a SeNP-based bio-nanocomposite with improved antioxidant activity.
KW - Alteromonas macleodii Mo 169
KW - bioactive material
KW - blue biotechnology
KW - exopolysaccharide (EPS)
KW - selenium nanoparticles (SeNP)
UR - http://www.scopus.com/inward/record.url?scp=85149067745&partnerID=8YFLogxK
U2 - 10.3390/bioengineering10020193
DO - 10.3390/bioengineering10020193
M3 - Article
C2 - 36829687
AN - SCOPUS:85149067745
SN - 2306-5354
VL - 10
JO - Bioengineering
JF - Bioengineering
IS - 2
M1 - 193
ER -
