Selective Activity of an Anthocyanin-Rich, Purified Blueberry Extract upon Pathogenic and Probiotic Bacteria

Sara Silva, Eduardo M. Costa, Manuela Machado, Rui M. Morais, Conceição Calhau, Manuela Pintado

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
23 Downloads (Pure)


Blueberry extracts have been widely recognized as possessing antimicrobial activity against several potential pathogens. However, the contextualization of the interaction of these extracts with beneficial bacteria (i.e., probiotics), particularly when considering the food applications of these products, may be of importance, not only because their presence is important in the regular gut microbiota, but also because they are important constituents of regular and functional foodstuffs. Therefore, the present work first sought to demonstrate the inhibitory effect of a blueberry extract upon four potential food pathogens and, after identifying the active concentrations, evaluated their impact upon the growth and metabolic activity (organic acid production and sugar consumption) of five potential probiotic microorganisms. Results showed that the extract, at a concentration that inhibited L. monocytogenes, B. cereus, E. coli and S. enteritidis (1000 μg mL−1), had no inhibitory effect on the growth of the potential probiotic stains used. However, the results demonstrated, for the first time, that the extract had a significant impact on the metabolic activity of all probiotic strains, resulting in higher amounts of organic acid production (acetic, citric and lactic acids) and an earlier production of propionic acid.

Original languageEnglish
Article number734
Issue number4
Publication statusPublished - Feb 2023


  • antimicrobial activity
  • blueberry extract
  • organic acids
  • pathogen
  • probiotic
  • short-chain fatty acids


Dive into the research topics of 'Selective Activity of an Anthocyanin-Rich, Purified Blueberry Extract upon Pathogenic and Probiotic Bacteria'. Together they form a unique fingerprint.

Cite this