Abstract
The present invention refers to an acceleration process for macromolecular fragmentation in liquid medium, in gel or in a solid support, by means of joint application of enzymes and ultrasounds, as well as isotopes. The macromolecules, in any of these liquids, are mixed with enzymes and inserted in a recipient (2) and exposed to an ultrasonic radiation field (1). The combined effect of the enzymes and of the ultrasounds allows accomplishing, in time ranges between 10 s and 600 s, the fragmentation of macromolecules or whole proteomes, with the correspondent formation of their constituent lower molecular units.
| Original language | English |
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| Patent number | WO2007007267 |
| IPC | C12Q1/00,G01N33/68 |
| Priority date | 7/07/05 |
| Filing date | 7/07/06 |
| Publication status | Published - 5 Jul 2007 |