Screening and characterization of multidrug-resistant gram-negative bacteria from a remote African Area, São Tomé and Príncipe

Laurent Poirel, Marta Aires-de-Sousa, Patrick Kudyba, Nicolas Kieffer, Patrice Nordmann

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The occurrence of resistance to last-resort antibiotics was evaluated among Enterobacteriaceae isolates recovered from hospitalized children in a remote African archipelago, São Tomé and Príncipe, where there is limited access to those antibiotics. Fifty patients were screened for colonization by carbapenem-, pan-aminoglycoside-, or polymyxin-resistant Enterobacteriaceae. A total of 36 isolates (including 30 Escherichia coli and 4 Klebsiella pneumoniae) were recovered from 23 patients, including 26 isolates harboring the blaOXA-181 carbapenemase gene, a single isolate harboring the 16S rRNA methylase gene rmtB encoding pan-resistance to aminoglycosides, and 8 isolates coharboring both genes. A single isolate possessed the plasmid-borne colistin resistance gene mcr-1. A high clonal relationship was found for OXA-181-producing E. coli (4 clones), and conversely, three of the four OXA-181-producing K. pneumoniae isolates were clonally unrelated. This study overall showed a high prevalence of resistance to last-resort antibiotics in this country, where no epidemiological data were previously available.

Original languageEnglish
Article numbere01021-18
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • Aminoglycosides
  • Carbapenem
  • Carbapenemase
  • Colistin
  • MCR
  • São Tomé and Príncipe

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