Rubredoxins derivatives

Simple sulphur-rich coordination metal sites and its relevance for biology and chemistry

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

Rubredoxins (Rds) and their derivatives have been extensively used, in the last few decades, in order to elucidate structure and functional aspects of metal sites involving rich sulphur coordination spheres. The derivatives have been designed with two main purposes: (a) replacement of selective and specific amino acid residues in native systems by site direct mutagenesis, and (b) replacement of native metal ion (iron), producing novel metal sites. We will highlight in this review the key amino acid residues, recognized in native Rds, directly involved in electron transfer mechanisms. By protein template assisted synthesis, metal-substituted Rds are used as structural probes and bio-models. The tetra-cysteinyl metal coordination site in Rd has the surprising capacity of chelating a wide variety of metal ions (other than native iron), and this chemistry has been witnessing significant growth based on two main interests: (i) metals such as 57Fe, Zn, Co, Cd, Ga, In, and Hg were introduced and derivatives were synthesized in order to specifically probe structural determinants imposed by the protein, and (ii) Zn, Ni, Cu, as well as Mo (and most recently, W), were used with a particular interest: to model complex metal centres in sulphur-rich enzymes. The latter, in particular, is discussed in the context of Rd serving as a model complex of native enzymes, as well as the synthesis of small inorganic complexes. The review aims to bring synthetic biochemistry and synthetic inorganic chemistry together in a synergistic way.

Original languageEnglish
Pages (from-to)379-397
Number of pages19
JournalCoordination Chemistry Reviews
Volume352
DOIs
Publication statusPublished - Dec 2017

Fingerprint

Rubredoxins
biology
Sulfur
sulfur
Metals
chemistry
Derivatives
metals
Amino acids
Iron
Enzymes
amino acids
enzymes
Proteins
Amino Acids
Mutagenesis
inorganic chemistry
Biochemistry
proteins
mutagenesis

Keywords

  • Derivatives
  • Electron transfer
  • Model chemistry
  • Probes
  • Rubredoxins

Cite this

@article{846c3518899348fd93b9f0238da56b62,
title = "Rubredoxins derivatives: Simple sulphur-rich coordination metal sites and its relevance for biology and chemistry",
abstract = "Rubredoxins (Rds) and their derivatives have been extensively used, in the last few decades, in order to elucidate structure and functional aspects of metal sites involving rich sulphur coordination spheres. The derivatives have been designed with two main purposes: (a) replacement of selective and specific amino acid residues in native systems by site direct mutagenesis, and (b) replacement of native metal ion (iron), producing novel metal sites. We will highlight in this review the key amino acid residues, recognized in native Rds, directly involved in electron transfer mechanisms. By protein template assisted synthesis, metal-substituted Rds are used as structural probes and bio-models. The tetra-cysteinyl metal coordination site in Rd has the surprising capacity of chelating a wide variety of metal ions (other than native iron), and this chemistry has been witnessing significant growth based on two main interests: (i) metals such as 57Fe, Zn, Co, Cd, Ga, In, and Hg were introduced and derivatives were synthesized in order to specifically probe structural determinants imposed by the protein, and (ii) Zn, Ni, Cu, as well as Mo (and most recently, W), were used with a particular interest: to model complex metal centres in sulphur-rich enzymes. The latter, in particular, is discussed in the context of Rd serving as a model complex of native enzymes, as well as the synthesis of small inorganic complexes. The review aims to bring synthetic biochemistry and synthetic inorganic chemistry together in a synergistic way.",
keywords = "Derivatives, Electron transfer, Model chemistry, Probes, Rubredoxins",
author = "Maiti, {Biplab K.} and Almeida, {Rui M.} and Isabel Moura and Moura, {Jos{\'e} J.G.}",
note = "This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO/REQUIMTE, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). BKM and RMA wish to thank FCT/MEC for the fellowship grants SFRH/BPD/63066/2009 and SERH/BPD/80293/2011, which are financed by national funds and co-financed by FSE.",
year = "2017",
month = "12",
doi = "10.1016/j.ccr.2017.10.001",
language = "English",
volume = "352",
pages = "379--397",
journal = "Coordination Chemistry Reviews",
issn = "0010-8545",
publisher = "Elsevier Science",

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TY - JOUR

T1 - Rubredoxins derivatives

T2 - Simple sulphur-rich coordination metal sites and its relevance for biology and chemistry

AU - Maiti, Biplab K.

AU - Almeida, Rui M.

AU - Moura, Isabel

AU - Moura, José J.G.

N1 - This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO/REQUIMTE, which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). BKM and RMA wish to thank FCT/MEC for the fellowship grants SFRH/BPD/63066/2009 and SERH/BPD/80293/2011, which are financed by national funds and co-financed by FSE.

PY - 2017/12

Y1 - 2017/12

N2 - Rubredoxins (Rds) and their derivatives have been extensively used, in the last few decades, in order to elucidate structure and functional aspects of metal sites involving rich sulphur coordination spheres. The derivatives have been designed with two main purposes: (a) replacement of selective and specific amino acid residues in native systems by site direct mutagenesis, and (b) replacement of native metal ion (iron), producing novel metal sites. We will highlight in this review the key amino acid residues, recognized in native Rds, directly involved in electron transfer mechanisms. By protein template assisted synthesis, metal-substituted Rds are used as structural probes and bio-models. The tetra-cysteinyl metal coordination site in Rd has the surprising capacity of chelating a wide variety of metal ions (other than native iron), and this chemistry has been witnessing significant growth based on two main interests: (i) metals such as 57Fe, Zn, Co, Cd, Ga, In, and Hg were introduced and derivatives were synthesized in order to specifically probe structural determinants imposed by the protein, and (ii) Zn, Ni, Cu, as well as Mo (and most recently, W), were used with a particular interest: to model complex metal centres in sulphur-rich enzymes. The latter, in particular, is discussed in the context of Rd serving as a model complex of native enzymes, as well as the synthesis of small inorganic complexes. The review aims to bring synthetic biochemistry and synthetic inorganic chemistry together in a synergistic way.

AB - Rubredoxins (Rds) and their derivatives have been extensively used, in the last few decades, in order to elucidate structure and functional aspects of metal sites involving rich sulphur coordination spheres. The derivatives have been designed with two main purposes: (a) replacement of selective and specific amino acid residues in native systems by site direct mutagenesis, and (b) replacement of native metal ion (iron), producing novel metal sites. We will highlight in this review the key amino acid residues, recognized in native Rds, directly involved in electron transfer mechanisms. By protein template assisted synthesis, metal-substituted Rds are used as structural probes and bio-models. The tetra-cysteinyl metal coordination site in Rd has the surprising capacity of chelating a wide variety of metal ions (other than native iron), and this chemistry has been witnessing significant growth based on two main interests: (i) metals such as 57Fe, Zn, Co, Cd, Ga, In, and Hg were introduced and derivatives were synthesized in order to specifically probe structural determinants imposed by the protein, and (ii) Zn, Ni, Cu, as well as Mo (and most recently, W), were used with a particular interest: to model complex metal centres in sulphur-rich enzymes. The latter, in particular, is discussed in the context of Rd serving as a model complex of native enzymes, as well as the synthesis of small inorganic complexes. The review aims to bring synthetic biochemistry and synthetic inorganic chemistry together in a synergistic way.

KW - Derivatives

KW - Electron transfer

KW - Model chemistry

KW - Probes

KW - Rubredoxins

UR - http://www.scopus.com/inward/record.url?scp=85033593559&partnerID=8YFLogxK

U2 - 10.1016/j.ccr.2017.10.001

DO - 10.1016/j.ccr.2017.10.001

M3 - Review article

VL - 352

SP - 379

EP - 397

JO - Coordination Chemistry Reviews

JF - Coordination Chemistry Reviews

SN - 0010-8545

ER -