Rubredoxin as a paramagnetic relaxation-inducing probe

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12 Citations (Scopus)

Abstract

The paramagnetic effect due to the presence of a metal center with unpaired electrons is no longer considered a hindrance in protein NMR spectroscopy. In the present work, the paramagnetic effect due to the presence of a metal center with unpaired electrons was used to map the interface of an electron transfer complex. Desulfovibrio gigas cytochrome c3 was chosen as target to study the effect of the paramagnetic probe, Fe-rubredoxin, which produced specific line broadening in the heme IV methyl resonances M21 and M181. The rubredoxin binding surface in the complex with cytochrome c3 was identified in a heteronuclear 2D NMR titration. The identified heme methyls on cytochrome c3 are involved in the binding interface of the complex, a result that is in agreement with the predicted complexes obtained by restrained molecular docking, which shows a cluster of possible solutions near heme IV. The use of a paramagnetic probe in 1HNMR titration and the mapping of the complex interface, in combination with a molecular simulation algorithm proved to be a valuable strategy to study electron transfer complexes involving non-heme iron proteins and cytochromes.

Original languageEnglish
Pages (from-to)1245-1253
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume103
Issue number9
DOIs
Publication statusPublished - 1 Sep 2009

Keywords

  • Electron transfer complex
  • Heme proteins
  • Paramagnetism
  • Relaxation
  • Rubredoxin

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