Role of vitamin B 12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri

J. M. Wood, Isabel Moura, José J. G. Moura, M. H. Santos, A. V. Xavier, J. LeGall, M. Scandellari

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


When Methanosarcina barkeri is grown on methanol as the sole carbon source, a B 12 -containing protein is synthesized by this organism. This B 12 protein contains bound aquocobalamin, and when this cofactor is reduced and methylated with [ 14 C]methyl iodide, the resultant [ 14 C]methyl B 12 protein is extremely active in the biosynthesis of 14 C-labeled methane. These findings indicate that a B 12 -dependent system is operative in the biological formation of methane in addition to other systems that are B 12 -independent.

Original languageEnglish
Pages (from-to)303-305
Number of pages3
Issue number4543
Publication statusPublished - 1 Jan 1982


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